Fluorogenic Protein-Based Strategies for Detection, Actuation, and Sensing

BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Arnaud Gautier, Alison G Tebo

Abstract

Fluorescence imaging has become an indispensable tool in cell and molecular biology. GFP-like fluorescent proteins have revolutionized fluorescence microscopy, giving experimenters exquisite control over the localization and specificity of tagged constructs. However, these systems present certain drawbacks and as such, alternative systems based on a fluorogenic interaction between a chromophore and a protein have been developed. While these systems are initially designed as fluorescent labels, they also present new opportunities for the development of novel labeling and detection strategies. This review focuses on new labeling protocols, actuation methods, and biosensors based on fluorogenic protein systems.

References

Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
Jun 27, 2000·Journal of Bacteriology·B J FeilmeierG J Phillips
Dec 7, 2002·Nature Biotechnology·Antje KepplerKai Johnsson
Apr 3, 2003·Nature Reviews. Cancer·S Perwez HussainCurtis C Harris
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Antje KepplerKai Johnsson
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Oct 27, 2006·Current Opinion in Structural Biology·S James Remington
Mar 14, 2007·Nature Biotechnology·Thomas DrepperKarl-Erich Jaeger
Apr 17, 2007·Molecular Cell·Elizabeth A VealBrian A Morgan
Dec 25, 2007·Nature Biotechnology·Christopher Szent-GyorgyiAlan Waggoner
Jan 12, 2008·The EMBO Journal·Valentin JaumouilléGuy Tran Van Nhieu
Feb 23, 2008·Chemistry & Biology·Arnaud GautierKai Johnsson
Jun 7, 2008·ACS Chemical Biology·Georgyi V LosKeith V Wood
Dec 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sean ChapmanJohn M Christie
Nov 3, 2009·Journal of the American Chemical Society·Yuichiro HoriKazuya Kikuchi
May 11, 2010·Applied and Environmental Microbiology·Jaroslaw E KrólEva M Top
Jan 1, 2009·Bioconjugate Chemistry·James A J FitzpatrickMarcel P Bruchez
Jul 19, 2011·Nature Biotechnology·Grigory S FilonovVladislav V Verkhusha
Jan 4, 2012·Angewandte Chemie·Grigory S FilonovVladislav V Verkhusha
Mar 31, 2012·Angewandte Chemie·Anmol GroverMarcel P Bruchez
Apr 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yingchuan B QiYishi Jin
Jan 25, 2013·Nature Chemistry·Gražvydas LukinavičiusKai Johnsson
May 11, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thomas DrepperMartina Pohl
Jun 19, 2013·Cell·Akiko KumagaiAtsushi Miyawaki
Jun 19, 2013·Nature Methods·Daria M Shcherbakova, Vladislav V Verkhusha
Jul 31, 2013·Chemistry & Biology·Grigory S Filonov, Vladislav V Verkhusha
Aug 10, 2013·Journal of the American Chemical Society·Yuichiro HoriKazuya Kikuchi
Nov 7, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Qi YanMarcel P Bruchez
Feb 7, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Marcus WingenThomas Gensch
Apr 22, 2014·Nature Methods·Emmanuelle TchekandaStephen W Michnick
Oct 29, 2014·Journal of Microbiology and Biotechnology·Yuvaraj RavikumarHyungdon Yun

❮ Previous
Next ❯

Citations

Jul 13, 2019·Biochemical Society Transactions·Ilijana VojnovicUlrike Endesfelder
Feb 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thorsten G MüllerBarbara Müller
Jun 8, 2021·Biochemistry·Luke D Lavis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Methods in Molecular Biology
George H Patterson
Journal of Biophotonics
Richard Wombacher, Virginia W Cornish
Methods : a Companion to Methods in Enzymology
P T TranFred Chang
© 2022 Meta ULC. All rights reserved