Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii

Applied and Environmental Microbiology
Se-Wook Oh, Dong-Hyun Kang

Abstract

4-Methylumbelliferyl-alpha-D-glucoside, the fluorogenic substrate of alpha-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.

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Citations

Apr 15, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Nicole T SehnemMarco A Záchia Ayub
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