Fluorometric detection of biological S-nitrosothiols

Analytical Biochemistry
J K Park, P Kostka

Abstract

A technique is presented for the quantitative detection of S-nitrosothiols formed by model biological thiols, cysteine, glutathione, and serum albumin. The technique is based on the detection of fluorescent compound 1-[H]-naphthotriazole formed between 2,3-diaminonaphthalene and nitrous acid released from S-nitrosothiols by treatment with mercuric chloride in an acidic environment. Concentration of S-nitrosothiols is determined from the difference in fluorescent signal (excitation/emission wavelengths of 363 nm/450 nm, respectively) observed in the presence and absence of 0.18 mM HgCl2. The yield of the reaction between 2,3-diaminonaphthalene and nitrous acid released from the S-NO bond by HgCl2 approaches 90-100% as documented by simultaneous assays of S-nitrosothiols by uv spectrophotometry and by Saville method. The assay can be applied to the analysis of mixtures containing excess of thiol and/or nitrite at neutral pH by pretreatment of samples with N-ethylmaleinimide and/or ammonium sulfamate, respectively. In analysis of S-nitrosothiols in protein-containing mixtures, HgCl2-mediated release of nitrous acid in the presence of 2,3-diaminonaphthalene is followed by neutralization of samples and precipitation of protein with ...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Jan 1, 1987·Annals of the New York Academy of Sciences·S R Tannenbaum, J S Wishnok
Jan 1, 1987·Methods in Enzymology·P C Jocelyn
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Nov 24, 1995·The Journal of Biological Chemistry·V G KharitonovV S Sharma
Oct 1, 1993·Analytical Biochemistry·T P MiskoM G Currie
Oct 1, 1994·The Journal of Clinical Investigation·J S ScharfsteinJ Loscalzo
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B GastonD J Singel
Mar 29, 1993·FEBS Letters·P Girard, P Potier
Jul 1, 1996·Analytical Biochemistry·J A CookD A Wink

❮ Previous
Next ❯

Citations

Feb 12, 2010·ACS Chemical Biology·Erika BechtoldS Bruce King
Nov 9, 2000·The European Journal of Neuroscience·T E SaltK Q Do
Dec 1, 2011·Molecular & Cellular Proteomics : MCP·Christopher I MurrayJennifer E Van Eyk
Feb 24, 2012·Antioxidants & Redox Signaling·Erika Bechtold, S Bruce King
Apr 3, 2001·American Journal of Respiratory and Critical Care Medicine·M CorradiP J Barnes
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·N RamachandranB Mutus
Jun 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shirin AkhterBulent Mutus
Jan 30, 2004·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Kenneth Wong, Xuebin Li
Jul 1, 1998·Clinical Biochemistry·G EllisS K Makela
Jul 11, 2014·Redox Biology·Sylvie RiquierJean-Claude Drapier
Feb 27, 2015·Scientific Reports·Fei XiongNing Gu
Sep 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dimitrios Tsikas
Oct 3, 2008·Journal of Neuroscience Research·Jorge M Romero, Oscar A Bizzozero
Jun 22, 2002·Annals of the New York Academy of Sciences·K Q DoT E Salt
Feb 16, 2006·Journal of Fluorescence·Ana GomesJosé L F C Lima
May 10, 2007·The Journal of Biological Chemistry·Eric R SchreiterJoseph Bonaventura
Aug 5, 2003·Canadian Journal of Physiology and Pharmacology·Ella S M Ng, Paul Kubes
Apr 28, 1999·Journal of Cardiovascular Pharmacology·P KostkaE H Skiles
May 1, 2009·Journal of Neuroscience Research·Oscar A Bizzozero, Jianzheng Zheng
Apr 16, 1998·The Journal of Biological Chemistry·S AleryaniP Kostka
Nov 21, 2002·The Journal of Biological Chemistry·Markus KnippMilan Vasák
Aug 6, 2000·Free Radical Biology & Medicine·N Hogg
Aug 5, 2003·Nitric Oxide : Biology and Chemistry·Shirin AkhterBulent Mutus
Sep 25, 2007·Journal of Inorganic Biochemistry·Julie Laterreur, Ann M English
Sep 3, 2021·Journal of Cellular Biochemistry·Ajanta Chatterji, Rajib Sengupta
Nov 4, 2008·Free Radical Biology & Medicine·Michael T ForresterJonathan S Stamler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.