Abstract
An F0F1-ATPase-based aptasensor is described for the fluorometric determination of Vibrio parahaemolyticus. Chromatophores containing F0F1-ATPases were first prepared from Rhodospirillum rubrum cells. Then, an aptamer-functionalized chromatophore acts as the capture probe, and a chromatophore labeled with the pH probe fluorescein acts as the signalling probe. In the presence of V. parahaemolyticus, the rotation rate of F0F1-ATPase is decreased due to the formation of the aptamer-chromatophore complex. This leads to a retarded proton flux out of the chromatophores. As a result, the pH value inside the chromatophores is reduced, and the fluorescence of the pH probe F1300 is accordingly decreased. The relative fluorescence varies linearly over the 15 to 1.5 × 106 cfu·mL-1 Vibrio parahaemolyticus concentration range, and the limit of detection is 15 cfu·mL-1. The method was applied to analyze artificially contaminated salmon samples where it showed excellent perfomance. Graphical abstract In this assay, aptamer functionalized chromatophores act as a capture probe, and the fluoresce in labeled chromatophores as signalling probe. The formation of aptamer-chromatophore complex leads to a retarded proton flux out of the chromatophores....Continue Reading
References
Aug 30, 1990·Nature·A D Ellington, J W Szostak
Aug 3, 1990·Science·C Tuerk, L Gold
Jan 22, 2005·Annual Review of Medicine·Shahid M NimjeeBruce A Sullenger
Jul 5, 2005·Foodborne Pathogens and Disease·P S Marie Yeung, Kathryn J Boor
Apr 10, 2007·Food Microbiology·Yi-Cheng Su, Chengchu Liu
Jun 18, 2011·Journal of the American Chemical Society·Ronit FreemanItamar Willner
Aug 16, 2011·Nucleic Acids Research·Ngoc Quang DoAnh Tuân Phan
Apr 7, 2012·Journal of Agricultural and Food Chemistry·Nuo DuanZhouping Wang
Jun 5, 2012·Biochemical and Biophysical Research Communications·Yueliang ZhaoYiming Ha
Jan 31, 2013·Journal of Microbiological Methods·Jie ZhangGuangquan Chen
Jul 25, 2014·Nature Protocols·Ying WangJinghong Li
Apr 23, 2015·Nature Chemistry·Ankita ShastriXimin He
Aug 25, 2015·Journal of Agricultural and Food Chemistry·Shijia WuZhouping Wang
Aug 15, 2017·Journal of Hazardous Materials·Ruojie ChengGuohua Zhao
Jan 1, 2016·Biotechnology and Bioprocess Engineering : BBE·Zhuo ZhaoHai-Yan Wu
Citations
Aug 30, 2020·Mikrochimica Acta·Wenting WeiXiurong Su
Jan 12, 2019·Mikrochimica Acta·Qiming QiuYibin Ying
Jun 12, 2019·Mikrochimica Acta·Shijia WuZhouping Wang
Mar 19, 2021·ACS Infectious Diseases·Carly DeusenberyAnita Shukla