PMID: 6397183Nov 1, 1984Paper

Fluorometric microassay of trypsin and enteropeptidase in children--comparison with a titrimetic assay

Australian Paediatric Journal
J D Mitchell, I M Messina

Abstract

Two trypsin assay methods for the estimation of this enzyme in duodenal fluid from children have been compared. Assay results for a fluorometric method based on the use of N-carbobenzoxy-diglycyl-L-arginyl-2-naphthylamide hydrochloride (GANA) as the trypsin substrate were found to correlate well (r = 0.91, P less than 0.001) with those obtained with a much less sensitive titrimetric assay which used benzoylarginine ethylester hydrochloride (BAEE) as substrate. The higher sensitivity of the fluorometric assay has allowed accurate determination of trypsin activity in 10 microliter aliquots of duodenal fluid. This low volume requirement makes the assay suitable for studies on infants of all ages and conserves duodenal fluid for use in other investigations often warranted during the assessment of childhood malabsorption. The fluorometric assay has also been used to monitor the separation of enteropeptidase from trypsin(ogen) by chromatography on Sephacryl S-200 in samples of duodenal fluid from two children. Different proteolytic pathway deficiencies were confirmed in these children.

References

Apr 19, 1969·Lancet·B HadornO H Wolff
Jul 31, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·H RinderknechtM C Geokas
Oct 1, 1970·Archives of Disease in Childhood·M J TarlowJ K Lloyd
Nov 1, 1964·Archives of Biochemistry and Biophysics·M M NACHLASA M SELIGMAN
Feb 1, 1965·The Journal of Pediatrics·P L TOWNES
Aug 1, 1967·Gut·H S Wiggins

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Citations

Jan 1, 1990·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M Szereda-PrzestaszewskaA Stránsky
Jan 1, 1985·Annals of the New York Academy of Sciences·R T BartusC Flicker

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