Fluoromycobacteriophages Can Detect Viable Mycobacterium tuberculosis and Determine Phenotypic Rifampicin Resistance in 3-5 Days From Sputum Collection

Frontiers in Microbiology
Liliana RondónMariana Piuri

Abstract

The World Health Organization (WHO) estimates that 40% of tuberculosis (TB) cases are not diagnosed and treated correctly. Even though there are several diagnostic tests available in the market, rapid, easy, inexpensive detection, and drug susceptibility testing (DST) of Mycobacterium tuberculosis is still of critical importance specially in low and middle-income countries with high incidence of the disease. In this work, we have developed a microscopy-based methodology using the reporter mycobacteriophage mCherrybomb ϕ for detection of Mycobacterium spp. and phenotypic determination of rifampicin resistance within just days from sputum sample collection. Fluoromycobacteriophage methodology is compatible with regularly used protocols in clinical laboratories for TB diagnosis and paraformaldehyde fixation after infection reduces biohazard risks with sample analysis by fluorescence microscopy. We have also set up conditions for discrimination between M. tuberculosis complex (MTBC) and non-tuberculous mycobacteria (NTM) strains by addition of p-nitrobenzoic acid (PNB) during the assay. Using clinical isolates of pre-XDR and XDR-TB strains from this study, we tested mCherrybomb Φ for extended DST and we compared the antibiotic resi...Continue Reading

References

Aug 1, 1997·Antimicrobial Agents and Chemotherapy·S SreevatsanJ M Musser
Jul 3, 1998·Journal of Clinical Microbiology·S A WattersonF A Drobniewski
Jan 25, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M E FordG F Hatfull
Feb 3, 2007·Health Technology Assessment : HTA·J DinnesA Lalvani
Jun 6, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ben J MaraisRob M Warren
Feb 13, 2009·PLoS Medicine·Andreas SandgrenMegan B Murray
Mar 31, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Gail E LouwThomas C Victor
May 12, 2009·Journal of Microbiological Methods·Anandi MartinJuan Carlos Palomino
Jul 23, 2009·PloS One·David N BreslauerDaniel A Fletcher
Jan 19, 2010·Bioinformatics·Heng Li, Richard Durbin
May 28, 2010·Journal of Clinical Microbiology·Robert BlakemoreDavid Alland
Jun 10, 2010·Annual Review of Microbiology·Graham F Hatfull
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Feb 25, 2011·Journal of Clinical Microbiology·Liliana RondónHoward E Takiff
Jun 15, 2011·FEMS Immunology and Medical Microbiology·Pedro Eduardo Almeida da SilvaJuan Carlos Palomino
Mar 1, 2012·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Nanda HemvaniD S Chitnis
Mar 17, 2012·Advances in Virus Research·Graham F Hatfull
Mar 6, 2013·Seminars in Respiratory and Critical Care Medicine·Philippe GlaziouMario Raviglione
Apr 1, 2011·Asian Pacific Journal of Tropical Biomedicine·Juan GarberiAlcides Troncoso
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Babak JavidEric J Rubin
Mar 22, 2014·Journal of Clinical Microbiology·Syed Beenish RufaiSarman Singh
May 13, 2014·Database : the Journal of Biological Databases and Curation·Leandro RaduskyAdrián G Turjanski
May 1, 2015·Journal of Clinical Microbiology·Max R O'DonnellMichelle H Larsen
May 12, 2015·Nature Communications·Vegard EldholmFrancois Balloux

❮ Previous
Next ❯

Citations

Sep 30, 2020·Annual Review of Virology·Graham F Hatfull
Feb 6, 2020·Biological & Pharmaceutical Bulletin·Javier Pizarro-Bauerle, Hiroki Ando
Jan 17, 2021·International Journal of Molecular Sciences·Anna Allué-GuardiaJordi B Torrelles
Jan 26, 2022·ACS Synthetic Biology·Anooshay KhanUrartu Ozgur Safak Seker

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA438689

Methods Mentioned

BETA
phage assay
fluorescence
flow cytometry
fluorescence microscopy
electrophoresis

Software Mentioned

MASS
mCherry
VCFKit
GATK
Hmisc
R
BWA
ETE Toolkit
SnpEff
PRINSEQ lite

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.