Fluorophore-gold nanoparticle complex for sensitive optical biosensing and imaging

Nanotechnology
Jianting WangKyung A Kang

Abstract

Fluorophores have been extensively used as the signal mediator in biosensing and bioimaging for a long time. Enhancement of fluorescence can amplify the signal, thus improving the sensitivity, enabling earlier and accurate disease detection and diagnosis. Some metal nanoparticles, such as gold and silver, can generate a strong electromagnetic field on their surface (surface plasmon field) upon receiving photonic energy. When a fluorophore is placed in the field, the field can affect the fluorophore electrons participating in fluorescence emission and change the fluorescence output. The change can be from complete quenching to significant enhancement, depending on the metal type, particle size and shape, excitation/emission wavelengths and quantum yield of the fluorophore, and the distance between the fluorophore and the particle surface. In this study, the effects of these parameters on the fluorescence enhancement of commonly used fluorophores by gold nanoparticles (GNPs) are theoretically analyzed. Experimentally, an NIR contrast agent with enhanced fluorescence was developed by carefully tailoring the distance between Cypate (ICG based fluorophore) and a GNP, via biocompatible spacer constructs. The effect of the GNP size (3...Continue Reading

References

Jan 1, 1978·Physics in Medicine and Biology·R C Benson, H A Kues
Aug 18, 2000·Investigative Radiology·S AchilefuR Rajagopalan
Apr 3, 2001·Nature Biotechnology·B DubertretA J Libchaber
Jun 26, 2003·Journal of the American Chemical Society·Yunpeng YeSamuel Achilefu
Jan 20, 2005·Bioconjugate Chemistry·Yunpeng YeSamuel Achilefu
Feb 5, 2005·Analytical Biochemistry·Joseph R Lakowicz
Apr 14, 2005·Chemical Reviews·J Christopher LoveGeorge M Whitesides
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ravi ShuklaMurali Sastry
Jan 19, 2006·Current Opinion in Chemical Biology·Mark Ming-Cheng ChengMauro Ferrari
Apr 12, 2006·Physical Review Letters·Pascal AngerLukas Novotny
Aug 16, 2006·Physical Review Letters·Sergei KühnVahid Sandoghdar
Jan 16, 2008·Small·Juan B González-DíazMikael Käll
Sep 2, 2008·Nature Reviews. Drug Discovery·Mark E DavisDong M Shin
Nov 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Laurence DuchesneDavid G Fernig
Oct 17, 2007·Optics Express·Palash Bharadwaj, Lukas Novotny
Mar 30, 2011·Advances in Experimental Medicine and Biology·Jianting WangKyung A Kang
May 17, 2011·Journal of Nanobiotechnology·Kyung A KangSamuel Achilefu
May 24, 2011·Analytica Chimica Acta·Jianting WangKyung A Kang

❮ Previous
Next ❯

Citations

Feb 14, 2013·Journal of the American Chemical Society·Stacy L CapehartMatthew B Francis
Mar 22, 2014·International Journal of Nanomedicine·Xiaomin YiJianlin Yuan
Apr 10, 2014·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Kyung A Kang, Jianting Wang
Sep 15, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Suprit DeolYoung-Seok Shon
May 18, 2016·Journal of Nanobiotechnology·M CordeiroP M V Baptista
Oct 12, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Jian WangZhou Jiang
Jun 21, 2018·Scientific Reports·Keying XuYidong Yang
Apr 4, 2020·ACS Applied Materials & Interfaces·Soundharraj PrabhaSingaravelu Ganesan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.