Fluoroquinolone-resistant and extended-spectrum beta-lactamase producing Escherichia coli isolates from free-living wild animals

Veterinary Microbiology
Maja VelhnerCorinna Kehrenberg

Abstract

During the hunting season 2013-2014, fecal samples collected from hare, roe deer, deer and wild boars were sent to the bacteriology laboratory for the isolation of Escherichia coli and multidrug resistant isolates were characterized phenotypically and genotypically. Out of 106 fecal samples, E. coli was isolated from 101 samples. Although the majority of isolates belonged to phylogenetic groups A and B1, 14 out of 101 isolates were affiliated to group B2. A multidrug resistance phenotype was determined in 7 isolates, all of which had distinguishable genomic macrorestriction profiles. PCR analysis and sequencing revealed a variety of resistance genes, gene cassettes and cassette arrays in these multidrug resistant isolates. Resistance to fluoroquinolones was found in five E. coli isolates (two from a roe deer, one from a deer and two from a wild boar) and multiple mutations in the chromosomal topoisomerase genes were identified. In an E.coli isolate from a hare, the qnrB19 gene was detected. The same isolate carried an aadA23 gene cassette in class 1 integron. In addition, an extended- spectrum beta-lactamase blaCTX-M-1 gene was detected in an E. coli isolate from a roe deer. The gene was located on a conjugative multi resistanc...Continue Reading

Citations

Sep 9, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Chanika PungpianRungtip Chuanchuen
Apr 30, 2020·Animals : an Open Access Journal From MDPI·Fabrizio BertelloniBarbara Turchi
Feb 16, 2021·Frontiers in Veterinary Science·Carolina Plaza-RodríguezBernd-Alois Tenhagen
Mar 7, 2021·Microorganisms·Anna R HoltmannCorinna Kehrenberg
May 28, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rana ElshimyRania Abdelmonem Khattab

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