Fluprostenol activates phospholipase C and Ca2+ mobilization in human myometrial cells

The Journal of Clinical Endocrinology and Metabolism
M P CarrascoA López Bernal

Abstract

The objective of this study was to investigate the mechanism of action of PGF2 alpha in cultured human myometrial cells. We measured the effects of PGF2 alpha and fluprostenol, a selective PGF2 alpha receptor (FP receptor) agonist, on phospholipase C(PLC) activation, on changes in the intracellular free calcium concentration ([Ca2+]i), and on protein tyrosine phosphorylation. PGF2 alpha and fluprostenol activated PLC (determined by measuring the formation of inositol phosphates) and increased [Ca2+]i in a concentration-dependent manner. The apparent affinity of the FP receptor for fluprostenol was higher than that for PGF2 alpha when measuring PLC activation, but the receptor displayed similar affinities for both agonists when measuring increases in [Ca2+]i. These effects were not altered by treating the cells with pertussis toxin (PT), suggesting that the FP receptor is linked to PLC activation by a G protein of the Gq family. By contrast, the effect of oxytocin on PLC activation involved both PT-resistant and PT-sensitive pathways. Human myometrial cells responded to pervanadate and epidermal growth factor with increased PLC activity and increased tyrosine phosphorylation, demonstrating a functional PLC-gamma tyrosine kinase ...Continue Reading

Citations

Apr 21, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Andrew M BlanksSteven Thornton
Mar 8, 2005·Experimental Cell Research·Glynis ScottAlice Pentland
Feb 20, 2007·American Journal of Obstetrics and Gynecology·Mark PhillippeDaniel Engle
Sep 19, 2000·American Journal of Obstetrics and Gynecology·M A ElovitzM Phillippe
Feb 27, 2003·Biology of Reproduction·Emmanuelle DallotMichelle Breuiller-Fouché
Jan 24, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gertrude-E Costin, Vincent J Hearing

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