Fluroescence of proteins in 6-M guanidine hydrochloride. A method for the quantitative determination of tryptophan
To determine the tryptophan content in proteins,an analytical ultraviolet fluroescence method is proposed based on making uniform the environment of aromatic chromophores in 6-7 M guanidine hydrochloride. The fluorescence intensity scale is calibrated using standard solutions of free tryptophan. A correlation coefficient between the fluorescence of protein tryptophanyl residues and of free tryptophan was estimated in testing 17 well characterized proteins. This method is particularly suited to proteins carrying groups absorbing in the 290-370 nm region, such as flavin, heme and pyridoxal phosphate and in the presence of substances such as 2-mercaptoethanol which prohibit the use of the spectroscopic or magnetic circular dichroism methods. It is less time-consuming than techniques requiring hydrolysis or chemical reactions.
Baker's yeast crystalline cytochrome b 2 : improved preparation and electrophoresis studies of subunit constitution
Magnetic circular dichroism studies. XVII. Magnetic circular dichroism spectra of proteins. A new method for the quantitative determination of tryptophan
Molecular weight and quaternary structure of yeast L-lactate dehydrogenase (cytochrome b2). 2. Revised heme extinction coefficients and minimal molecular weight
Reconstitution of cytochrome b2 following prosthetic groups dissociation by guanidine hydrochloride, Protoheme binding
Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A
Fluorescence and the structure of proteins. I. Effects of substituents on the fluorescence of indole and phenol compounds
FLUORESCENCE AND THE STRUCTURE OF PROTEINS. II. FLUORESCENCE OF PEPTIDES CONTAINING TRYPTOPHAN OR TYROSINE
Stability and reconstitution of pyruvate oxidase from Lactobacillus plantarum: dissection of the stabilizing effects of coenzyme binding and subunit interaction
On the lack of coordination between protein folding and flavin insertion in Escherichia coli for flavocytochrome b2 mutant forms Y254L and D282N
Selective labeling of alpha-bungarotoxin with fluorescein isothiocyanate and its use for the study of toxin-acetylcholine receptor interactions
Studies on the chemical modification of goat liver cystatin and the effect on its anti-papain inhibitory activity
Purification and comparative properties of NADPH-cytochrome P-450 reductase from rat and rainbow trout: differences in temperature optima between reconstituted and microsomal trout enzymes
Direct evidence for the alterations in protein structure and conformation upon in vitro nonenzymatic glycosylation
Mutation to glutamine of histidine 373, the catalytic base of flavocytochrome b2 (L-lactate dehydrogenase)
Structural and functional properties of Drosophila melanogaster phosphorylase: comparison with the rabbit skeletal muscle enzyme
Perfluoroalkylphosphocholines are poor protein-solubilizing surfactants, as tested with neutrophil plasma membranes
Mimicking lipid-binding-induced conformational changes in the human apolipoprotein E N-terminal receptor binding domain effects of low pH and propanol
Flavin-protein interactions in flavocytochrome b2 as studied by NMR after reconstitution of the enzyme with 13C- and 15N-labelled flavin
Morphological changes in rat pancreatic slices associated with inhibition of enzyme secretion by high concentrations of secretagogues
Purification and characterization of the DNA cleavage and recognition site of I-ScaI mitochondrial group I intron encoded endonuclease produced in Escherichia coli
Peroxisomal copper, zinc superoxide dismutase. Characterization of the isoenzyme from watermelon cotyledons
Structural studies of yeast flavocytochrome b2: cooperative roles of the alpha and beta globules in the formation of the flavin-binding sites
The "b5-like" domain from chicken-liver sulfite oxidase: a new case of common ancestral origin with liver cytochrome b5 and bakers' yeast cytochrome b2 core
Second-derivative spectroscopy of proteins. A method for the quantitative determination of aromatic amino acids in proteins
Studies on polypeptide-chain-elongation factors from an extreme thermophile, Thermus thermophilus HB8. 3. Molecular properties
A study of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Improved purification, relative molecular mass, and amino acid composition
Purification and characterization of multiplication-stimulating activity. Insulin-like growth factors purified from rat-liver-cell-conditioned medium
Pyridine nucleotide transhydrogenase from Azotobacter vinelandii. Improved purification, physical properties and subunit arrangement in purified polymers
Spontaneous dissociation of a cytochrome core and a biglobular flavoprotein after mild trypsinolysis of the bifunctional Saccharomyces cerevisiae flavocytochrome b2
Proteolytic cleavage of Hansenula anomala flavocytochrome b2 into its two functional domains. Isolation of a highly active flavodehydrogenase and a cytochrome b2 core
Physicochemical characterization of a fast refolding monomeric class I fructose-1,6-bisphosphate aldolase from Staphylococcus aureus
Primary structure of flavocytochrome b2 from baker's yeast. Purification by reverse-phase high-pressure liquid chromatography and sequencing of fragment alpha cyanogen bromide peptides
The composition of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Does a unifying model exist for the complexes from gram-negative bacteria?
Juvenile-hormone-binding protein from the hemolymph of Galleria mellonella (L). Isolation and characterization
The domain structure of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii
Amino-acid sequence of the cytochrome-b5-like heme-binding domain from Hansenula anomala flavocytochrome b2
Lipoamide dehydrogenase from Azotobacter vinelandii. Molecular cloning, organization and sequence analysis of the gene
Amino acid sequence determination and three-dimensional modelling of thioredoxin from the photosynthetic bacterium Rhodobacter sphaeroides Y
Isolation of the flavodehydrogenase domain of Hansenula anomala flavocytochrome b2 after mild proteolysis by an H. anomala proteinase
Purification and biochemical characterization of a putative [6Fe-6S] prismane-cluster-containing protein from Desulfovibrio vulgaris (Hildenborough)
The primary structure of a protein containing a putative [6Fe-6S] prismane cluster from Desulfovibrio vulgaris (Hildenborough)
The DNA-binding domain of the yeast Saccharomyces cerevisiae CYP1(HAP1) transcription factor possesses two zinc ions which are complexed in a zinc cluster
The role of a beta barrel loop 4 extension in modulating the physical and functional properties of long-chain 2-hydroxy-acid oxidase isozymes
Some DNA targets of the yeast CYP1 transcriptional activator are functionally asymmetric--evidence of two half-sites with different affinities
Tryptophanyl fluorescence quenching of urocanase from Pseudomonas putida by acrylamide, cesium, iodide, and imidazolepropionate
Amino acid sequence of an unique ribonuclease with a C-terminus rich in O-glycosylated serine and threonine from culture medium of Lentinus edodes
Influence of the stability of a fused protein and its distance to the amyloidogenic segment on fibril formation
Characterization and quantification of intact 26S proteasome proteins by real-time measurement of intrinsic fluorescence prior to top-down mass spectrometry
Under proper control, oxidation of proteins with known chemical structure provides an accurate and absolute method for the determination of their molar concentration
The effect of radiation-induced reactive oxygen species (ROS) on the structural and functional properties of yeast alcohol dehydrogenase (YADH)
On prilling of hydrophilic microgels in lipid dispersions using mono-N-carboxymethyl chitosan for oral biologicals delivery
A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus
Tryptophan fluorescence reveals the presence of long-range interactions in the denatured state of ribonuclease Sa
The structure of human apolipoprotein E2, E3 and E4 in solution. 2. Multidomain organization correlates with the stability of apoE structure
A comparative analysis of the fluorescence properties of the wild-type and active site mutants of the hepatitis C virus autoprotease NS2-3
New (15)N NMR exchange experiments for the unambiguous assignment of (1)H(N)/(15)N resonances of proteins in complexes in slow chemical exchange with free form
The COX18 gene, involved in mitochondrial biogenesis, is functionally conserved and tightly regulated in humans and fission yeast
Unfolding diminishes fluorescence resonance energy transfer (FRET) of lysine modified β-lactoglobulin: Relevance towards anti-HIV binding
Isolation and characterization of independently folding regions of the beta chain of Escherichia coli tryptophan synthetase
Rapid kinetic studies of partial reactions in the heme free derivative of L-lactate cytochrome c oxidoreductase (flavocytochrome b2); the flavodehydrogenase function
Spectroscopic investigation of ligand interaction with hepatic phenylalanine hydroxylase: evidence for a conformational change associated with activation
Chemical characterization of lumazine protein from Photobacterium leiognathi: comparison with lumazine protein from Photobacterium phosphoreum
Rat kidney L-2-hydroxyacid oxidase. Structural and mechanistic comparison with flavocytochrome b2 from baker's yeast
Intrinsic tryptophan fluorescence of Schizosaccharomyces pombe mitochondrial F1-ATPase. A powerful probe for phosphate and nucleotide interactions
The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study
Controlled proteolysis of flavocytochrome b2. Characterization of a 15000-dalton heme-binding core and comparison with detergent solubilized cytochrome b5
Enzyme memory. 2. Kinetics and thermodynamics of the slow conformation changes of wheat-germ hexokinase LI
Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria
Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species
A flavin-mononucleotide-binding site in Hansenula anomala nicked flavocytochrome b2, requiring the association of two domains
Deconvolution of the fluorescence emission spectrum of human antithrombin and identification of the tryptophan residues that are responsive to heparin binding.
Studies on the properties of highly purified cytochrome P-448 and its dependent activity benzo[a]pyrene hydroxylase, from Saccharomyces cerevisiae
Effects of segment substitution on the structure and stability of immunoglobulin G binding domain of streptococcal protein G
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