PMID: 6113534Jan 1, 1981Paper

Flurorcitrate resistant tricarboxylate transport mutants of Salmonella typhimurium

Molecular & General Genetics : MGG
J M SomersW W Kay

Abstract

Spontaneous and Tn10 induced fluorocitrate resistant mutants were isolated and characterized. These mutants were unable to grow on either cis-aconitate or DL-isocitrate but were still able to grow slowly on sodium citrate and normally on potassium or potassium-plus-sodium citrate. These mutants were defective in both citrate transport and citrate binding to priplasmic proteins. Tn10 insertion mutants were unable to produce immunologically detectable amounts of the citrate inducible periplasmic C protein previously shown to bind tricarboxylates. Using a series of tct::Tn10 directed Hfrs the tct locus was accurately positioned at 59 units between srlA and pheA, but was not cotransducible with either gene. In the absence of P22 mediated cotransduction with 16 adjacent chromosomal markers the srlA and tct loci were bridged by using a series of tct flanking Tn10 insertions, and by newly isolated and characterized nalB mutants. In addition the hyd and recA loci were located establishing the gene order in this region of the chromosome as: pheA tct nalB recA srlA hyd cys. Nitrosoguanidine derived tricarboxylate mutations (Imai 1975) were also mapped within the tct locus.

References

Dec 1, 1979·Journal of Bacteriology·T D Ingolia, D E Koshland
Sep 1, 1978·Archives of Biochemistry and Biophysics·W W Kay, M Cameron
Oct 1, 1979·Journal of Bacteriology·M Kihara, R M Macnab
Oct 5, 1975·Journal of Molecular Biology·N KlecknerD Botstein
Nov 30, 1973·Biochimica Et Biophysica Acta·G E Frost, H Rosenberg

❮ Previous
Next ❯

Citations

Jan 1, 1983·Molecular & General Genetics : MGG·J M Somers, W W Kay
Sep 23, 2003·Research in Microbiology·Brit WinnenMilton H Saier
Aug 23, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J WainG Frankel
Oct 10, 2018·Environmental Microbiology Reports·Isotta D'ArrigoKatherine S Long
Feb 27, 2018·Frontiers in Cellular and Infection Microbiology·Leonardo T RosaDavid J Kelly
Mar 23, 2021·Critical Reviews in Microbiology·Lucindo Cardoso de PinaL Caetano M Antunes
Sep 1, 1983·Microbiological Reviews·K E Sanderson, J R Roth
Dec 1, 1983·Journal of Bacteriology·C H Reynolds, S Silver

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.