Flux control of the malate valve in leaf cells

Archives of Biochemistry and Biophysics
L E FridlyandR Scheibe

Abstract

The coupled processes of the chloroplast trans-envelope transport of malate and oxaloacetate and their interconversion as catalyzed by the stromal NADP-linked malate dehydrogenase are quantitatively analyzed by means of a steady-state model. The equation for the NADP-malate dehydrogenase reaction is developed. The empirical dependence of enzyme activity on NADPH and NADP+ is used to determine its actual activity. The trans-envelope counter exchange of malate and oxaloacetate is described by a kinetic model of the translocator. Kinetic parameters are derived from known data, except for the Km value and the maximum rate for oxaloacetate transport, which are estimated from oxaloacetate-dependent malate formation in isolated intact chloroplasts. Using the kinetic properties of the system and the known metabolite concentrations, the model demonstrates that photosynthetically generated NADPH can be exported efficiently from the chloroplasts to the cytosol by the malate-valve system. The transfer capacity of the malate valve is estimated not to exceed 20 mumol (mg Chl)-1 h-1 (or 5% of the electron transport) under normal physiological conditions. The possible role of the malate valve in leaf cells under normal conditions and during st...Continue Reading

References

Mar 13, 1978·Biochimica Et Biophysica Acta·K Lehner, H W Heldt
Oct 1, 1991·European Journal of Biochemistry·R HeinrichH G Holzhütter
Apr 1, 1991·Plant Physiology·D HeinekeH W Heldt
Jan 1, 1987·Photosynthesis Research·M Vivekanandan, G E Edwards

❮ Previous
Next ❯

Citations

Apr 5, 2011·Journal of Experimental Botany·Mitsue MiyaoHiroshi Fukayama
Mar 1, 2012·Journal of Experimental Botany·Christine H FoyerJeremy Harbinson
Oct 17, 2007·Plant & Cell Physiology·Agu LaiskRenate Scheibe
Feb 15, 2008·Plant & Cell Physiology·Gabriela Leticia MüllerMaría Valeria Lara
Aug 23, 2005·Plant Physiology·Mariel C Gerrard WheelerVerónica G Maurino
Jan 11, 2007·Annual Review of Plant Biology·Ken-ichiro ShimazakiToshinori Kinoshita
Jan 15, 2010·The Plant Journal : for Cell and Molecular Biology·Amy Marshall ColónJohn Morgan
Dec 12, 2012·Plant Physiology and Biochemistry : PPB·Mariana SaigoMaría F Drincovich
Feb 18, 2012·Current Opinion in Plant Biology·Mitsutaka Taniguchi, Hiroshi Miyake
Jun 15, 2007·Phytochemistry·Rigoberto Rios-Estepa, Bernd Markus Lange
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Petra RennéAndreas P M Weber
Mar 23, 2004·Physiologia Plantarum·Renate Scheibe
Jul 31, 2007·Plant, Cell & Environment·Neil R BakerDavid M Kramer
Oct 12, 2010·Bio Systems·Abir U Igamberdiev, Leszek A Kleczkowski
Jan 28, 2015·Biochimica Et Biophysica Acta·Bettina BölterSerena Schwenkert
Jan 22, 2002·Metabolic Engineering·John A Morgan, David Rhodes
Mar 11, 2018·Biochemical Society Transactions·Elmien Heyneke, Alisdair R Fernie
Jun 23, 2018·Plant Biology·J Selinski, R Scheibe
Jun 16, 2006·Plant & Cell Physiology·Agu LaiskHeikko Rämma
Oct 24, 2019·Annals of Botany·Alexandrina StirbetGovindjee Govindjee
Dec 13, 2017·Nature Plants·Florian A BuschGraham D Farquhar
Dec 8, 2006·The Journal of Biological Chemistry·Enrique DetarsioMaría F Drincovich
Oct 26, 2012·Molecular Biology Reports·Qiguo YuYuxiang Cheng
Apr 12, 2020·Mitochondrion·Abir U Igamberdiev
Mar 20, 2021·Journal of Experimental Botany·Thomas Wieloch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.