FMAj: a tool for high content analysis of muscle dynamics in Drosophila metamorphosis

BMC Bioinformatics
KuleeshaMartin Wasser

Abstract

During metamorphosis in Drosophila melanogaster, larval muscles undergo two different developmental fates; one population is removed by cell death, while the other persistent subset undergoes morphological remodeling and survives to adulthood. Thanks to the ability to perform live imaging of muscle development in transparent pupae and the power of genetics, metamorphosis in Drosophila can be used as a model to study the regulation of skeletal muscle mass. However, time-lapse microscopy generates sizeable image data that require new tools for high throughput image analysis. We performed targeted gene perturbation in muscles and acquired 3D time-series images of muscles in metamorphosis using laser scanning confocal microscopy. To quantify the phenotypic effects of gene perturbations, we designed the Fly Muscle Analysis tool (FMAj) which is based on the ImageJ and MySQL frameworks for image processing and data storage, respectively. The image analysis pipeline of FMAj contains three modules. The first module assists in adding annotations to time-lapse datasets, such as genotypes, experimental parameters and temporal reference points, which are used to compare different datasets. The second module performs segmentation and feature...Continue Reading

References

Oct 4, 2015·Methods : a Companion to Methods in Enzymology·Wee Choo Puah, Martin Wasser
May 5, 2016·BMC Developmental Biology· KuleeshaMartin Wasser
Dec 19, 2014·BMC Bioinformatics·Shoba RanganathanChristian Schönbach
Jul 5, 2018·Cell Death & Disease·Kumar VishalErika R Geisbrecht

Citations

Jun 21, 2006·The Journal of Cell Biology·Krista M HennigThomas P Neufeld
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Jan 20, 2010·FEBS Letters·Jemma L Webber, Sharon A Tooze
Mar 12, 2010·Nature·Frank SchnorrerBarry J Dickson
Jul 30, 2011·Skeletal Muscle·Stefano Schiaffino, Cristina Mammucari
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jun 30, 2012·Nature Methods·Pasi KankaanpääDaniel J White
Jun 30, 2012·Nature Methods·Fabrice de ChaumontJean-Christophe Olivo-Marin
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Mar 2, 2013·PloS One·Brad T MooreL Ryan Baugh
Mar 23, 2013·The FEBS Journal·Stefano SchiaffinoMarco Sandri
Jun 1, 2013·Nature Methods·Johan HenrikssonThomas R Bürglin
Jul 31, 2013·Autophagy·Perrine Castets, Markus A Rüegg

Related Concepts

Biologic Segmentation
Myogenesis
Cosuppression
Muscle Cells
Drosophila melanogaster Proteins
Hypertrophic Cardiomyopathy
Science of Morphology
Myopathy
Hypertrophy
Drosophila

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