PMID: 6412560Sep 1, 1983Paper

FMLP-induced enzyme release from neutrophils: a role for intracellular calcium

The American Journal of Physiology
D E ChandlerC L Stapleton

Abstract

The ability of the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (FMLP) to stimulate beta-glucuronidase release and 45Ca2+ release from rabbit neutrophils was studied. FMLP stimulated enzyme release from cytochalasin B-treated cells either in the presence or the absence of extracellular calcium. Depletion of cell calcium, by exposure to either ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid or the calcium ionophore A23187, blocked the ability of FMLP to stimulate enzyme release and 45Ca2+ release in the absence of extracellular calcium. The ability of A23187 to lower the 45Ca2+ content of neutrophils, to block FMLP-stimulated 45Ca2+ release, and to inhibit FMLP-stimulated enzyme release in the absence of calcium was dose dependent over the same concentration range (10(-8) to 10(-6) M A23187) for all three actions. In contrast, FMLP stimulated enzyme release from A23187-treated cells, provided that extracellular calcium was present. This secretory response was normal as judged by cell ultrastructure and FMLP dose-response relationships. It is concluded that A23187 depletes a pool of intracellular calcium usually released by FMLP and that release of calcium from this pool is necessary for initiation of...Continue Reading

References

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Citations

Oct 3, 2002·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·P U ReberU Scheurer
Apr 21, 2005·Pancreas·Tomohiro Kobayashi, Yasuhiko Ohta
Sep 1, 1986·Digestive Diseases and Sciences·R K MittalR W McCallum
May 16, 2006·Digestive Diseases·Sangeeta R Mehendale, Chun-Su Yuan
Jun 1, 1989·British Poultry Science·C J SavoryM R Yeomans
Dec 28, 1999·European Journal of Pharmacology·H Z Lee, C H Wu
Jan 17, 1993·Biochimica Et Biophysica Acta·G J BoonenJ G Elferink
Aug 14, 1987·Biochemical and Biophysical Research Communications·B StyrtM S Klempner
Mar 1, 1989·Kidney International·F PuglieseG A Cinotti

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