Abstract
Within the hematopoietic compartment, fibromodulin (FMOD) is almost exclusively expressed in chronic lymphocytic leukemia (CLL) lymphocytes. We set out to determine whether FMOD could be of help in diagnosing borderline lymphoproliferative disorders (LPD). We established 3 flow cytometry-defined groups (CLL [n = 65], borderline LPD [n = 28], broadly defined as those with CLLflow score between 35 and -20 or discordant CD43 and CLLflow, and non-CLL LPD [n = 40]). FMOD expression levels were determined by standard RT-PCR in whole-blood samples. Patients were included regardless of lymphocyte count but with tumor burden ≥40%. FMOD expression levels distinguished between CLL (median 98.5, interquartile range [IQR] 37.8-195.1) and non-CLL LPD (median 0.012, IQR 0.003-0.033) with a sensitivity and specificity of 1. Most borderline LPDs were CD5/CD23/CD200-positive with no loss of B-cell antigens and negative or partial expression of CD43. 16/22 patients with available cytogenetic analysis showed trisomy 12. In 25/28 (89%) of these patients, FMOD expression levels fell between CLL and non-CLL (median 3.58, IQR 1.06-6.21). This study could suggest that borderline LPDs may constitute a distinct group laying in the biological spectrum of ...Continue Reading
References
Oct 10, 1997·American Journal of Clinical Pathology·E J MoreauD Catovsky
Aug 7, 2001·Reviews in Clinical and Experimental Hematology·E Matutes, A Polliack
Apr 3, 2003·British Journal of Haematology·Nicolas LeupinMartin F Fey
Mar 3, 2005·Blood·Eva MikaelssonHodjattallah Rabbani
Jan 26, 2007·Leukemia·N C GutiérrezJ F San Miguel
Dec 7, 2007·Cytometry. Part B, Clinical Cytometry·Sandra QuijanoAlberto Orfao
Feb 24, 2009·Leukemia Research·Giuseppe A PalumboFrancesco Di Raimondo
May 19, 2010·Blood·Marta SalidoFrancesc Solé
Apr 20, 2011·American Journal of Clinical Pathology·Yang O HuhLynne V Abruzzo
Dec 5, 2012·Bone Marrow Transplantation·Y Kanda
Nov 14, 2013·PloS One·Naoki MoriToshiko Motoji
Nov 19, 2013·Cytometry. Part B, Clinical Cytometry·Alex F SandesEdgar G Rizzatti
Jul 23, 2015·Nature·Xose S PuenteElías Campo
Sep 1, 2015·Immunologic Research·Brian A McCarthyNicholas Chiorazzi
May 20, 2017·Haematologica·Alba NavarroSílvia Beà
Aug 24, 2017·British Journal of Haematology·Thomas KöhnkeMarion Subklewe
Feb 10, 2018·Oncotarget·Shuhua YiLugui Qiu
Mar 16, 2018·Blood·Michael HallekThomas J Kipps
Jul 7, 2018·Haematologica·Lynne V AbruzzoKevin R Coombes
Oct 6, 2018·International Journal of Laboratory Hematology·Marc Sorigue, Jordi Junca
Dec 29, 2018·Cytometry. Part B, Clinical Cytometry·Marc SorigueJordi Juncà
Citations
Sep 23, 2020·Cytometry. Part B, Clinical Cytometry·Andrea EspasaMarc Sorigue
Dec 1, 2020·Expert Review of Hematology·Marc Sorigue
Jan 18, 2021·International Journal of Laboratory Hematology·Arambam GautamNeelam Varma
May 6, 2021·International Journal of Laboratory Hematology·Marc SorigueJordi Junca
Jul 5, 2021·International Journal of Laboratory Hematology·Marc SorigueLurdes Zamora