FMOD expression in whole blood aids in distinguishing between chronic lymphocytic leukemia and other leukemic lymphoproliferative disorders. A pilot study.

Cytometry. Part B, Clinical Cytometry
Marc SorigueLurdes Zamora

Abstract

Within the hematopoietic compartment, fibromodulin (FMOD) is almost exclusively expressed in chronic lymphocytic leukemia (CLL) lymphocytes. We set out to determine whether FMOD could be of help in diagnosing borderline lymphoproliferative disorders (LPD). We established 3 flow cytometry-defined groups (CLL [n = 65], borderline LPD [n = 28], broadly defined as those with CLLflow score between 35 and -20 or discordant CD43 and CLLflow, and non-CLL LPD [n = 40]). FMOD expression levels were determined by standard RT-PCR in whole-blood samples. Patients were included regardless of lymphocyte count but with tumor burden ≥40%. FMOD expression levels distinguished between CLL (median 98.5, interquartile range [IQR] 37.8-195.1) and non-CLL LPD (median 0.012, IQR 0.003-0.033) with a sensitivity and specificity of 1. Most borderline LPDs were CD5/CD23/CD200-positive with no loss of B-cell antigens and negative or partial expression of CD43. 16/22 patients with available cytogenetic analysis showed trisomy 12. In 25/28 (89%) of these patients, FMOD expression levels fell between CLL and non-CLL (median 3.58, IQR 1.06-6.21). This study could suggest that borderline LPDs may constitute a distinct group laying in the biological spectrum of ...Continue Reading

References

Oct 10, 1997·American Journal of Clinical Pathology·E J MoreauD Catovsky
Aug 7, 2001·Reviews in Clinical and Experimental Hematology·E Matutes, A Polliack
Apr 3, 2003·British Journal of Haematology·Nicolas LeupinMartin F Fey
Nov 19, 2013·Cytometry. Part B, Clinical Cytometry·Alex F SandesEdgar G Rizzatti
Jul 23, 2015·Nature·Xose S PuenteElías Campo
Aug 24, 2017·British Journal of Haematology·Thomas KöhnkeMarion Subklewe
Oct 6, 2018·International Journal of Laboratory Hematology·Marc Sorigue, Jordi Junca
Dec 29, 2018·Cytometry. Part B, Clinical Cytometry·Marc SorigueJordi Juncà

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Citations

Sep 23, 2020·Cytometry. Part B, Clinical Cytometry·Andrea EspasaMarc Sorigue
Dec 1, 2020·Expert Review of Hematology·Marc Sorigue
May 6, 2021·International Journal of Laboratory Hematology·Marc SorigueJordi Junca
Jul 5, 2021·International Journal of Laboratory Hematology·Marc SorigueLurdes Zamora

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