Abstract
The effect of the CSF-1 receptor, cFMS, on the phosphorylation of the retinoblastoma (RB) tumor suppressor protein and on the cell cycle and cell differentiation was analyzed in a cultured promyelocytic leukemia cell capable of induced myelomonocytic differentiation. A series of cFMS-transfected HL-60 sublines with progressively higher cell surface FMS expression was derived by flow cytometric cell sorting. Overexpression of FMS increased the duration of the cell cycle, prolonging all cell cycle phases especially S phase, which doubled. The increased cell cycle generation times occurred without any detectable changes in RB expression level or phosphorylation. For retinoic acid (RA)-induced myeloid differentiation, progressive overexpression of FMS caused a greater fraction of cells to differentiate and G1/0 arrest compared to wild-type cells after the same number of cell cycle generation times. FMS overexpression also progressively increased the relative amount of dephosphorylated RB protein induced, while reducing the total amount of RB protein. The inducer-originated and FMS-driven changes in RB hypophosphorylation were not effected through changes in p21/WAF1/CIP1 in this p53-negative cell. Similar effects on differentiation...Continue Reading
Citations
Oct 31, 2001·Differentiation; Research in Biological Diversity·H Y HongA Yen
Apr 27, 2004·Nucleic Acids Research·Hyun Jin Kwun, Kyung Lib Jang
May 3, 2001·Journal of Virology·A YenS Varvayanis
Mar 21, 2009·Experimental Cell Research·James SmithAndrew Yen
Oct 22, 2002·The Journal of General Virology·Ji Young AhnKyung Lib Jang
Feb 11, 1998·Experimental Cell Research·J D PlatkoA Yen
Aug 12, 2008·Experimental Cell Research·M Ariel KaussAndrew Yen
Jan 28, 2006·European Journal of Cell Biology·Andrew YenThomas J Lamkin
Jul 11, 2006·American Journal of Hematology·Thomas J LamkinAndrew Yen
May 25, 2001·International Journal of Hematology·S HagiwaraA Yuo
Sep 24, 2004·Molecular Pharmacology·Andrew YenSusi Varvayanis
Jul 17, 2004·Blood·Michael WitcherWilson H Miller
Jan 9, 1999·The Journal of Biological Chemistry·K J TownsendR W Craig
Jun 14, 2000·In Vitro Cellular & Developmental Biology. Animal·A Yen, S Varvayanis
May 8, 2020·Infectious Agents and Cancer·Shaian TavakolianEbrahim Faghihloo
May 1, 1999·Experimental Cell Research·A YenS Varvayanis