Fms-like tyrosine kinase 3 ligand regulates migratory pattern and antigen uptake of lung dendritic cell subsets in a murine model of allergic airway inflammation.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Zhifei ShaoDevendra K Agrawal

Abstract

Fms-like tyrosine kinase 3 ligand (Flt3L) reverses the features of allergic airway inflammation and increases a Th2-suppressive regulatory lung CD11c(high)CD11b(low) dendritic cell (DC) subset in a mouse model. We examined the migratory pattern and Ag uptake efficiency of lung DC subsets in the therapeutic effect of Flt3L. Lung CD11c(high)CD11b(low) and CD11c(low)CD11b(high) DCs from PBS-treated, OVA-sensitized, and Flt3L-treated/OVA-sensitized BALB/c mice were sorted using MACS and FACS for phenotype analysis. Lymphatic chemokine expression in thoracic lymph nodes was determined by immunohistochemistry. Migration of two lung DC subsets to lymphatic chemokines was examined in vitro using a Transwell chemotaxis assay. Labeled Ag was intranasally delivered into mouse lung to track the migration and Ag uptake of lung DCs. The in vitro cytokine secretion of mediastinal lymph node cells was determined using ELISA. CD11c(low)CD11b(high) DCs have higher expression of CCR5, CCR6, and CCR7, but lower expression of CCR2 than CD11c(high)CD11b(low) DCs. CD11c(low)CD11b(high) DCs in Flt3L-treated/OVA-sensitized mice demonstrated a less mature phenotype, inefficiency in Ag uptake, and impaired migration in vitro to lymphatic chemokine than t...Continue Reading

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Citations

Apr 29, 2010·Current Allergy and Asthma Reports·Devendra K Agrawal, Zhifei Shao
Apr 16, 2011·American Journal of Respiratory Cell and Molecular Biology·Zhifei ShaoDevendra K Agrawal
Dec 1, 2011·Journal of Visualized Experiments : JoVE·Wallissa Lancelin, Antonieta Guerrero-Plata
Jun 16, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K LeeJ Youn
Oct 17, 2013·Expert Review of Clinical Immunology·Rohit Gaurav, Devendra K Agrawal

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