FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites.

Microbiology
Wenmao MengJ R Guest

Abstract

The ndh gene of Escherichia coli encodes a non-proton-translocating NADH dehydrogenase (NdhII) that is anaerobically repressed by the global transcription regulator, FNR. FNR binds at two sites (centred at -50.5 and -94.5) in the ndh promoter but the mechanism of FNR-mediated repression appears not to be due to promoter occlusion. This mechanism has been investigated using an aerobically active derivative of FNR, FNR* (FNR-D154A), with ndh promoters containing altered FNR-binding sites. FNR* repressed ndh gene expression both aerobically and anaerobically in vivo. Gel retardation analysis and DNase I footprinting with purified FNR* protein confirmed that FNR interacts at two sites in the ndh promoter, and that FNR and RNA polymerase (RNAP) can bind simultaneously. Studies with three altered ndh promoters, each containing an impaired or improved FNR-site, indicated that both FNR-sites are needed for efficient repression in vivo. The alpha-subunit of RNAP interacted with two regions (centred at -105 and -46), each overlapping one of the FNR-sites in the ndh promoter. Footprints of the FNR*-RNAP-ndh ternary complex indicated that FNR*-binding at -50.5 prevents the alpha-subunit of RNAP from docking with the DNA just upstream of th...Continue Reading

References

Sep 23, 1991·Proceedings. Biological Sciences·A D SharrocksJ R Guest
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K IgarashiA Ishihama
Jan 1, 1991·Journal of Bacteriology·P J Kiley, W S Reznikoff
Sep 1, 1991·Microbiological Reviews·J Collado-VidesJ D Gralla
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Lanzer, H Bujard
Dec 4, 1987·Cell·S B Straney, D M Crothers
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Dec 1, 1995·Journal of Bacteriology·H J WingS J Busby
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N KhoroshilovaP J Kiley
Apr 1, 1993·Molecular Microbiology·J GreenJ R Guest
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S B Melville, R P Gunsalus
Jan 1, 1996·Molecular Microbiology·J GreenJ R Guest

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Citations

Jun 19, 1998·Biochimica Et Biophysica Acta·T YagiA Matsuno-Yagi
Jul 24, 1998·Trends in Microbiology·W Margolin
Oct 3, 1998·Trends in Biochemical Sciences·P L Graumann, M A Marahiel
Apr 3, 2001·Current Opinion in Microbiology·F Rojo
May 3, 2000·Molecular Microbiology·M V ColomboT Ruiz-Argüeso
Jul 3, 2002·The EMBO Journal·Hugo Cruz-RamosRobert K Poole
Oct 4, 2003·Journal of Bacteriology·Anne M L BarnardStephen J W Busby
Nov 18, 2004·Journal of Bacteriology·Victoria R SuttonPatricia J Kiley
Jun 5, 2007·Molecular Microbiology·Sandy M S WongBrian J Akerley
Nov 13, 2003·FEMS Microbiology Letters·Sarah L Messenger, Jeffrey Green
Jan 21, 2018·Cell Death and Differentiation·Zinaida DedeićXin Lu
Feb 10, 2004·Microbiology·Laura JacksonJeffrey Green
May 20, 2003·The Journal of Biological Chemistry·Kirsty SalmonRobert P Gunsalus
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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