Foam cell-derived 4-hydroxynonenal induces endothelial cell senescence in a TXNIP-dependent manner

Journal of Cellular and Molecular Medicine
Yael RiahiShlomo Sasson

Abstract

Vascular endothelial cell (VEC) senescence is considered an early event in the development of atherosclerotic lesions. Stressful stimuli, in particular oxidative stress, have been linked to premature senescence in the vasculature. Foam cells are a major source of reactive oxygen species and may play a role in the induction of VEC senescence; hence, we investigated their involvement in the induction of VEC senescence in a co-culture transwell system. Primary bovine aortic endothelial cells, exposed to the secretome of THP-1 monocyte-derived foam cells, were analysed for the induction of senescence. Senescence associated β-galactosidase activity and the expression of p16 and p21 were increased, whereas phosphorylated retinoblastoma protein was reduced. This senescent phenotype was mediated by 4-hydroxnonenal (4-HNE), a lipid peroxidation product secreted from foam cells; scavenging of 4-HNE in the co-culture medium blunted this effect. Furthermore, both foam cells and 4-HNE increased the expression of the pro-oxidant thioredoxin-interacting protein (TXNIP). Molecular manipulation of TXNIP expression confirmed its involvement in foam cell-induced senescence. Previous studies showed that peroxisome proliferator-activated receptor (...Continue Reading

References

Nov 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·K F Bürrig
Jan 25, 1996·The New England Journal of Medicine·F D Loop
Jan 14, 1999·The New England Journal of Medicine·R Ross
Jun 22, 1999·The New England Journal of Medicine·G D Sloop
Feb 7, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M FentonJ D Erusalimsky
Mar 6, 2002·Circulation·Peter LibbyAttilio Maseri
Mar 13, 2002·Circulation·Johann W AuerBernd Eber
Jun 19, 2002·Trends in Cardiovascular Medicine·Burkhard LudewigHans Hengartner
Sep 6, 2002·Biochemical Pharmacology·Jean-François DierickOlivier Toussaint
Nov 2, 2002·Nature Medicine·Andrew C Li, Christopher K Glass
Dec 4, 2003·Circulation Research·Hideyuki YamawakiBradford C Berk
Dec 31, 2003·Experimental Gerontology·Leonard Hayflick
Aug 4, 2004·Free Radical Biology & Medicine·Giuseppina BarreraMario U Dianzani
Sep 1, 2004·Free Radical Biology & Medicine·Dennis R Petersen, Jonathan A Doorn
Feb 8, 2005·The Journal of Clinical Investigation·Hideyuki YamawakiBradford C Berk
Apr 12, 2005·Cardiovascular Research·Ralf P BrandesRudi Busse
Nov 5, 2005·Molecular Nutrition & Food Research·Gabriella LeonarduzziGiuseppe Poli
Dec 20, 2005·Micron : the International Research and Review Journal for Microscopy·Yuri V Bobryshev
Feb 9, 2006·Journal of the American Chemical Society·Jianguo Fang, Arne Holmgren
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jun Chen, Michael S Goligorsky
Jun 8, 2006·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Uma Singh, Ishwarlal Jialal
Oct 6, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Cameron J WorldBradford C Berk
Jan 6, 2007·Circulation Research·Tohru Minamino, Issei Komuro
Nov 6, 2007·The American Journal of Pathology·Young-Mi GoDean P Jones
Nov 28, 2007·Annual Review of Pathology·Göran K HanssonCecilia Söderberg-Nauclér
Dec 7, 2007·Medicinal Research Reviews·G PoliG Leonarduzzi
Jun 17, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Hidetaka OtaYasuyoshi Ouchi
Oct 22, 2008·Pharmacology & Therapeutics·Toshio HayashiAkihisa Iguchi
Oct 23, 2008·Cell and Tissue Research·Wineke BakkerVictor W M van Hinsbergh
Nov 28, 2008·Journal of Applied Physiology·Jorge D Erusalimsky
Feb 4, 2009·Antioxidants & Redox Signaling·Joachim Altschmied, Judith Haendeler
Mar 26, 2009·Current Opinion in Hematology·Michael S GoligorskySusann Patschan
Oct 13, 2009·Journal of Cellular and Molecular Medicine·Anca V SimaMaya Simionescu

❮ Previous
Next ❯

Citations

Apr 5, 2016·The Canadian Journal of Cardiology·Hao Yin, J Geoffrey Pickering
Jan 1, 2016·Biochimie·R SalvayreC Camaré
Mar 26, 2016·Molecular Aspects of Medicine·Giuseppe MaulucciShlomo Sasson
Sep 27, 2016·Thrombosis Research·Magdalena L BochenekKatrin Schäfer
Dec 18, 2018·IUBMB Life·Jean-Marc Zingg
Dec 21, 2016·Oxidative Medicine and Cellular Longevity·Justyna Mikuła-PietrasikKrzysztof Książek
May 8, 2020·Cells·Nicole Wagner, Kay-Dietrich Wagner
Jul 22, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Chang-Meng WuYan-Wei Hu
Feb 5, 2021·Mechanisms of Ageing and Development·Jacopo SabbatinelliFabiola Olivieri
Feb 12, 2021·International Journal of Molecular Sciences·Alison DominguesValérie Nivet-Antoine
Aug 28, 2021·Antioxidants·Jean-Marc ZinggRoberta Ricciarelli

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA
transfection
coronary artery bypass
confocal microscopy

Software Mentioned

NIS
Clarity
GraphPad Prism
Nikon
Lite
Elements AR

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.