PMID: 9445065Jan 28, 1998Paper

Foamy virus particle formation

Journal of Virology
N FischerA Rethwilm

Abstract

Subgenomic expression plasmids for the so-called human foamy virus (HFV) structural gag, gag/pol, and env genes were constructed and used to analyze foamy virus particle formation by electron microscopy. Expression of an R-U5-gag-pol construct under control of the human cytomegalovirus immediate-early enhancer-promoter resulted in the formation of viral cores with a homogeneous size of approximately 50 nm located in the cytoplasm. Upon coexpression of an envelope construct, particles were observed budding into cytoplasmic vesicles and from the plasma membrane. Expression of the Gag protein precursor pr74 alone led to aberrantly formed viral particles of heterogeneous size and with open cores. Normal-shaped cores were seen after transfection of a construct expressing the p70gag cleavage product, indicating that p70gag is able to assemble into capsids. Coexpression of p70gag and Env resulted in budding virions, ruling out a requirement of the reverse transcriptase for capsid or virion formation. In sharp contrast to other retroviruses, the HFV cores did not spontaneously bud from cellular membranes. Radiochemical labeling followed by protein gel electrophoresis also revealed the intracellular retention of Env-deprived HFV capsids.

References

Jun 1, 1991·AIDS·J W Wills, R C Craven
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·V Bruss, D Ganem
May 1, 1990·The Journal of General Virology·K O NetzerV ter Meulen
Jan 1, 1987·Molecular and Cellular Biology·R B DuBridgeM P Calos
Jan 1, 1988·Advances in Cancer Research·E L Kuff, K K Lueders
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Oct 1, 1994·The Journal of General Virology·H HahnA Rethwilm
Aug 1, 1994·Journal of Virology·A W Schliephake, A Rethwilm
Sep 1, 1993·Trends in Microbiology·M Nassal, H Schaller
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M LöcheltR M Flügel
Sep 1, 1993·Journal of Virology·G BaunachA Rethwilm
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J EnssleA Rethwilm
Mar 21, 1996·Nature·R A Weiss
Jan 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·A Rethwilm
Jan 1, 1997·Journal of Virology·P A GoepfertM J Mulligan
Feb 1, 1997·Journal of Virology·M L GironR Emanoil-Ravier
Aug 28, 1997·Biochemical and Biophysical Research Communications·K I PfrepperR M Flügel

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Citations

Nov 15, 2006·Histochemistry and Cell Biology·Florian L P BenderMichael Sendtner
Mar 28, 2008·Journal of Virology·Melvyn W YapJonathan P Stoye
Aug 8, 2008·Journal of Virology·Annett StangeDirk Lindemann
Apr 12, 2013·Viruses·Sylvia HütterDirk Lindemann
Feb 23, 2000·Journal of Virology·T WilkM Löchelt
Jan 29, 2003·Journal of Virology·Kit L ShawPaul A Goepfert
May 12, 2005·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·S C BeutelspacherM O McClure
Jan 16, 2004·Gene Therapy·M Picard-MaureauM Heinkelein
Sep 15, 2005·Journal of Virology·Marc CartellieriAxel Rethwilm
May 7, 2010·Medical Microbiology and Immunology·Axel Rethwilm
Nov 18, 2011·Journal of Virology·Réjane RuaOlivier Schwartz
Mar 26, 2003·The Journal of General Virology·John A G BriggsStephen D Fuller
Dec 16, 1998·Human Gene Therapy·G D Trobridge, D W Russell
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maria ZhadinaPaul D Bieniasz
Mar 27, 2001·Journal of Virology·D CainM O McClure
Dec 17, 2010·Journal of Virology·Jochen BodemAxel Rethwilm
Feb 4, 2011·Journal of Virology·Daniel MatthesAxel Rethwilm
Sep 28, 2004·The Journal of General Virology·Gillian S PattonMyra O McClure
Mar 22, 2002·Journal of Virology·Martin HeinkeleinAxel Rethwilm
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Oct 12, 2012·Cellular Microbiology·Anka SwiersyDirk Lindemann
Apr 29, 2005·Journal of Virology·Gillian S PattonMyra O McClure
Sep 12, 2009·Expert Opinion on Biological Therapy·Grant D Trobridge
Sep 29, 2011·Nucleic Acids Research·Manuella BouttierCharles-Henri Lecellier
Oct 2, 2014·Retrovirology·Ben BerkhoutRobin A Weiss
Sep 3, 2016·Viruses·Martin V Hamann, Dirk Lindemann
May 28, 2005·Journal of Virology·Daniel LüfteneggerDirk Lindemann

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