Foamy virus vectors for suicide gene therapy

Gene Therapy
U NestlerA Rethwilm

Abstract

To improve the delivery of so-called suicide genes into tumors, recombinant retroviruses were constructed by inserting the herpes virus type 1 (HSV-1) thymidine kinase (tk), the E. coli cytosine deaminase (cd) and polynucleoside phosphorylase (pnp), or the jellyfish gene for the green fluorescent protein (gfp) into a foamy virus (FV)-derived replication-competent vector (pFOV-7). Expression and stability of the inserted foreign gene was analyzed by immunoblot and polymerase chain reaction (PCR). The functionality of the suicide genes was determined by a metabolic assay on virus vector infected cells and treatment with the respective prodrugs. In terms of vector stability and effectiveness of specific cell killing a virus transducing the pnp gene (FOV-7/pnp) was superior to those using the other two suicide genes. FOV-7/pnp is a candidate virus for suicide gene delivery into solid tumors.

Citations

May 5, 2001·Pharmacology & Therapeutics·A BzowskaD Shugar
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Dec 16, 1998·Human Gene Therapy·G D Trobridge, D W Russell
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Sep 12, 2009·Expert Opinion on Biological Therapy·Grant D Trobridge
Dec 29, 2005·Trends in Biotechnology·Pablo de FelipeMartin D Ryan
Apr 23, 2015·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E L RosenthalE J Sorscher
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Oct 9, 1999·Journal of Virology·M SchweizerD Neumann-Haefelin
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