Focal adhesion kinase is essential for costamerogenesis in cultured skeletal muscle cells

Developmental Biology
Navaline L Quach, Thomas A Rando

Abstract

A central question in muscle biology is how costameres are formed and become aligned with underlying myofibrils in mature tissues. Costameres are composed of focal adhesion proteins, including vinculin and paxillin, and anchor myofibril Z-bands to the sarcolemma. In the present study, we investigated the process of costamere formation ("costamerogenesis") in differentiating primary mouse myoblasts. Using vinculin and paxillin as costameric markers, we found that two additional focal adhesion components, alpha5beta1 integrin and focal adhesion kinase (FAK), are associated with costameres. We have characterized costamerogenesis as occurring in three distinct stages based on the organizational pattern of these costameric proteins. We show that both costamerogenesis and myofibrillogenesis are initiated at sites of membrane contacts with the extracellular matrix and that their maturation is tightly coupled. To test the importance of FAK signaling in these processes, we analyzed cells expressing a dominant negative form of FAK (dnFAK). When cells expressing dnFAK were induced to differentiate, both costamerogenesis and myofibrillogenesis were disrupted although the expression of constituent proteins was not inhibited. Likewise, inhib...Continue Reading

References

Sep 1, 1992·The Journal of Cell Biology·B A DanowskiJ W Sanger
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K HanksS K Patel
Mar 1, 1991·Science·H F Epstein, D A Fischman
May 5, 1986·FEBS Letters·A M BelkinV E Koteliansky
Oct 1, 1986·The Journal of Cell Biology·J G TidballK Burridge
Jan 27, 1971·Nature: New Biology·J W SangerH Holtzer
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J V PardoS W Craig
Aug 8, 1983·FEBS Letters·V E Koteliansky, G N Gneushev
Jun 1, 1983·Developmental Biology·T K BorgP H Lill
Jul 21, 1995·The Journal of Biological Chemistry·S L BellisC E Turner
Jul 14, 1995·The Journal of Biological Chemistry·H C ChenJ L Guan
Jan 1, 1994·Cell Motility and the Cytoskeleton·D RheeJ W Sanger
Jul 1, 1993·Medicine and Science in Sports and Exercise·K KamiE Senba
Nov 1, 1995·In Vitro Cellular & Developmental Biology. Animal·J D RosenblattT A Partridge
Nov 14, 1997·Nature Genetics·U MayerK von der Mark
May 5, 1998·Neuromuscular Disorders : NMD·T A RandoA Franco
May 20, 1998·Nature Genetics·Y K HayashiK Arahata
Nov 13, 1998·The Journal of Cell Biology·D TavernaR O Hynes
Jul 17, 1999·The American Journal of Physiology·M FlückF W Booth
Nov 7, 1999·The Journal of Biological Chemistry·J G TidballE Lavergne
Apr 6, 2000·The Journal of Biological Chemistry·J M TaylorJ T Parsons
Aug 11, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·N H Brown
Aug 26, 2000·The Journal of Biological Chemistry·M LaserD Kuppuswamy
Nov 18, 2000·American Journal of Physiology. Cell Physiology·P G De Deyne
Jul 31, 2001·Biochimica Et Biophysica Acta·M D Schaller

❮ Previous
Next ❯

Citations

May 7, 2014·The Journal of Cell Biology·Stéphane VassilopoulosMarc Bitoun
Oct 15, 2014·BioMed Research International·Martin FlückThomas Gustafsson
Jun 17, 2014·Rejuvenation Research·Nuria GaratacheaAlejandro Lucia
Mar 29, 2014·Histochemistry and Cell Biology·Oliver J WilsonAnton J M Wagenmakers
Nov 23, 2006·BMC Developmental Biology·Mira D LozykMichal Opas
Sep 28, 2007·Molecular Biology of the Cell·Mei ZhangAlan R Saltiel
Jul 10, 2008·BMC Cell Biology·James J DowlingEva L Feldman
Jul 5, 2011·The Journal of Biological Chemistry·Elizabeth P EwenFrancisco J Naya
Jun 12, 2012·PLoS Computational Biology·Benjamin M FriedrichSamuel A Safran
Dec 28, 2011·Journal of Materials Science. Materials in Medicine·José Ballester-BeltránManuel Salmerón-Sánchez
Jul 3, 2013·Skeletal Muscle·Christopher J StorbeckLuc A Sabourin
Jul 4, 2012·Histochemistry and Cell Biology·Oliver J WilsonAnton J M Wagenmakers
Jun 8, 2013·Biotechnology and Bioengineering·José Ballester-BeltránManuel Salmerón-Sánchez
May 28, 2009·The Journal of Physiology·Anne-Cécile DurieuxMartin Flück
Jul 2, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Huei-Ju Ting, Chawnshang Chang
Jan 18, 2015·Developmental Biology·Aidan P Maartens, Nicholas H Brown
Jun 28, 2011·Scandinavian Journal of Medicine & Science in Sports·M FlueckM Narici
Jun 28, 2011·Scandinavian Journal of Medicine & Science in Sports·M V NariciE Mueller
Sep 10, 2013·Journal of Anatomy·Ruowei LiMartin Flück
Jul 30, 2008·Biochemical and Biophysical Research Communications·Luciene BorgesSara Teresinha Olalla Saad
Jul 5, 2015·Journal of Muscle Research and Cell Motility·Zachary A GrahamChristopher P Cardozo
Aug 5, 2015·Tissue Engineering. Part a·Patricia RicoManuel Salmerón-Sánchez
Jun 20, 2015·Expert Reviews in Molecular Medicine·Oihane JakaAmets Sáenz
Dec 15, 2010·Molecular Cell·Stéphane C BoutetThomas A Rando
Mar 6, 2012·Cell Stem Cell·Stéphane C BoutetThomas A Rando
Apr 28, 2007·Journal of Molecular Biology·Katja GehmlichDieter O Fürst
Mar 11, 2011·PLoS Computational Biology·Anna GrosbergKevin Kit Parker
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Dan LuoThomas A Rando
Jul 30, 2014·Scientific Reports·Aleixandre Rodrigo-NavarroManuel Salmeron-Sanchez
Apr 26, 2018·American Journal of Physiology. Cell Physiology·Andrea A DomenighettiRichard L Lieber
Dec 22, 2006·Cell Motility and the Cytoskeleton·Melissa A SenetarRichard O McCann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.