Abstract
Tubulobulbar complexes (TBCs) are actin-related double-membrane invaginations formed at intercellular junctions in the seminiferous epithelium of mammalian testis. They occur at basal junction complexes between neighboring Sertoli cells and at apical junctions between Sertoli cells and spermatids. They are proposed to internalize intercellular junctions during the translocation of spermatocytes from basal to adluminal compartments of the seminiferous epithelium, and during sperm release from Sertoli cells. Although TBCs are specific to the seminiferous epithelium, they morphologically resemble podosomes in osteoclasts. Previously, we have reported that a key group of proteins consisting of N-WASp, Arp2/3, cortactin and dynamin that occur at podosomes also is present at TBCs. Here we explore the prediction that zyxin, a focal adhesion protein known to be present at podosomes, also is present at apical TBCs. A rabbit polyclonal anti-zyxin antibody (B71) was used to label fixed fragments and frozen sections of testis. In both fragments and sections, B71 labeled tubular regions of TBCs at apical sites of attachment between Sertoli cells and spermatids, in addition to being localized at actin related intercellular adhesion junctions...Continue Reading
References
Jun 1, 1979·The American Journal of Anatomy·L D Russell
Jul 1, 1976·The Anatomical Record·L Russell, Y Clermont
Oct 21, 1994·Cell·K L Schmeichel, M C Beckerle
Sep 8, 1997·The Journal of Cell Biology·D A Nix, M C Beckerle
May 10, 2000·The Journal of Biological Chemistry·B DreesR M Golsteyn
Oct 10, 2002·Experimental Cell Research·Chi-Ming HaiMario Gimona
Dec 17, 2002·Molecular and Cellular Biology·Laura M HoffmanMary C Beckerle
Jul 3, 2003·Trends in Cell Biology·Stefan Linder, Martin Aepfelbacher
Nov 20, 2003·Journal of Cell Science·Irina KaverinaMario Gimona
Feb 5, 2004·Journal of Andrology·Nikki P Y LeeC Yan Cheng
Apr 16, 2004·Biology of Reproduction·Julian A GuttmanA Wayne Vogl
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Roberto BuccioneMark A McNiven
Mar 8, 2005·Experimental Cell Research·Valérie AmsellemChristian Auclair
Jun 10, 2005·American Journal of Physiology. Cell Physiology·Bradley A WebbAlan S Mak
Oct 26, 2005·The Journal of Cell Biology·Masaaki YoshigiMary C Beckerle
Nov 30, 2006·Journal of Cellular Physiology·K S VaidA W Vogl
Sep 6, 2007·Cell Structure and Function·Norihiro KusumiKohji Takei
Sep 19, 2008·Biology of Reproduction·J'Nelle S YoungA Wayne Vogl
Sep 19, 2008·Biology of Reproduction·J'Nelle S YoungA Wayne Vogl
Aug 24, 2010·Nature Cell Biology·Min WuPietro De Camilli
Citations
May 15, 2013·Asian Journal of Andrology·Xiaojing QianC Yan Cheng
Jan 5, 2013·Human Reproduction Update·Xiang XiaoC Yan Cheng
Apr 9, 2013·PloS One·Xiaojing QianC Yan Cheng
Jun 7, 2012·Spermatogenesis·Elizabeth I TangC Yan Cheng
May 21, 2013·Spermatogenesis·Xiaojing QianC Yan Cheng
Apr 25, 2015·PloS One·Kira YoungCalvin P H Vary
Apr 5, 2013·PloS One·Xiujun ZhangMengchun Jia
Dec 16, 2016·Biochimica Et Biophysica Acta. Molecular Cell Research·Katarzyna ChojnackaDolores D Mruk
Oct 18, 2018·Cell Proliferation·Gaoming LiLiu Yang
Oct 13, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Arlo AdamsA Wayne Vogl
Jul 25, 2013·American Journal of Physiology. Endocrinology and Metabolism·Hin-Ting WanC Yan Cheng
Oct 18, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elissa W P WongC Yan Cheng
May 1, 2014·Spermatogenesis·C Yan Cheng
Jun 5, 2018·Reproduction, Fertility, and Development·A KumarN H Balasinor
Sep 20, 2017·Seminars in Cell & Developmental Biology·Haiqi ChenC Yan Cheng