Focal cerebral ischemic tolerance and change in blood-brain barrier permeability after repetitive pure oxygen exposure preconditioning in a rodent model

Journal of Neurosurgery
Xi WangXijing Zhang

Abstract

OBJECTIVE The goal of this study was to demonstrate that repetitive pure oxygen exposure preconditioning (O2PC) for 8 hours per day for 3 or 7 days, a practicable preconditioning for clinical use, is able to induce cerebral ischemic tolerance (IT) and further clarify the accompanying changes in the blood-brain barrier (BBB) that may be involved. METHODS A total of 68 adult male Sprague-Dawley rats and eight 1-day-old rat pups were used in this study. The adult rats were exposed to pure O2 (38 rats) 8 hours a day for 3 or 7 days or to room air (in an identical setup) for 8 hours a day for 7 days as controls (30 rats). Arterial O2 tension (PaO2) was measured in 6 rats exposed to O2 and 3 controls. Focal cerebral ischemia was elicited by middle cerebral artery occlusion (MCAO) in 37 rats, of which 21 had been exposed to pure O2 for 3 or 7 days and 16 to room air for 7 days as controls. Neurological behavior was scored with the Garcia score in 15 MCAO rats, of which 10 had been exposed to pure O2 for 3 or 7 days and 5 to room air for 7 days as controls, and cerebral infarct volumes were assessed with TTC (2,3,5-triphenyltetrazolium chloride) staining in 10 rats (5 from each group) after 7 days of exposure. Formamide-extraction meth...Continue Reading

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Citations

Oct 21, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ashley McDonough, Jonathan R Weinstein
Nov 8, 2017·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ashley McDonough, Jonathan R Weinstein
Apr 20, 2017·Molecular Neurobiology·Bruna BellaverAndré Quincozes-Santos
Jun 9, 2021·Neural Regeneration Research·Yuan ZhuGuo-Hui Jiang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
fluorescence microscopy

Software Mentioned

SPSS
Image
Pro Plus

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