Focal experimental autoimmune encephalomyelitis in the Lewis rat induced by immunization with myelin oligodendrocyte glycoprotein and intraspinal injection of vascular endothelial growth factor

Glia
Masanori SasakiJ D Kocsis

Abstract

Various models of experimental autoimmune encephalomyelitis (EAE) have led to insights into the pathogenesis and novel therapies for multiple sclerosis. One generalized EAE model uses immunizing the Lewis Rat with myelin oligodendrocyte glycoprotein (MOG) and complete Freund's adjuvant that induces systemic disease and inflammatory lesions at random central nervous system (CNS) locations. These lesions result from a combination of sensitized T cells and pathogenic antibodies gaining access to the CNS to cause an immune assault on myelin-expressing oligodendrocytes. We report a focal and temporal variant of the EAE model that results in immune-mediated demyelination at a predictable time and location. Lewis rats were immunized with the extracellular domain (1-125) of recombinant rat MOG in incomplete Freund's adjuvant (IFA) to induce a clinically silent humoral response. Vascular endothelial growth factor (VEGF) was then microinjected into the spinal cord to induce a transient, focal breakdown of the blood brain barrier (BBB). Clinical signs were apparent within 72 hours and began to resolve by day 21. The histopathology at the site of injection consisted of a focal region containing OX-42(+) cells, phagocytic cells with debris,...Continue Reading

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Citations

Jan 29, 2011·Molecular Neurobiology·Sreenivasan Ponnambalam, Mario Alberghina
Jul 16, 2011·Experimental & Translational Stroke Medicine·Anne WaschbischDe-Hyung Lee
Apr 27, 2011·Current Opinion in Neurology·Andrew R Pachner
Mar 31, 2012·Trends in Molecular Medicine·Osamu HonmouJeffery D Kocsis
Jan 1, 2012·Multiple Sclerosis and Related Disorders·Markus KippSandra Amor
Sep 6, 2011·Progress in Neurobiology·Minesh Kapadia, Boris Sakic
Dec 1, 2015·Journal of Neuroimmunology·Priya ChaudharyDennis Bourdette
Jul 23, 2014·Acta Neuropathologica Communications·Francesco GirolamoMaria Trojano
Apr 14, 2016·Experimental Neurology·Ian D Duncan, Abigail B Radcliff
Feb 2, 2011·Experimental Neurology·Masanori SasakiJeffery D Kocsis
Sep 18, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jang-Mi ParkMun-Yong Lee
Feb 16, 2018·Journal of Neuroscience Research·Ivana BjelobabaIrena Lavrnja
Oct 24, 2017·Current Protocols in Neuroscience·Kalliopi PitarokoiliRalf Gold

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