Focal exposure of limited lung volumes to high-dose irradiation down-regulated organ development-related functions and up-regulated the immune response in mouse pulmonary tissues

BMC Genetics
Bu-Yeo KimYun-Sil Lee

Abstract

Despite the emergence of stereotactic body radiotherapy (SBRT) for treatment of medically inoperable early-stage non-small-cell lung cancer patients, the molecular effects of focal exposure of limited lung volumes to high-dose radiation have not been fully characterized. This study was designed to identify molecular changes induced by focal high-dose irradiation using a mouse model of SBRT. Central areas of the mouse left lung were focally-irradiated (3 mm in diameter) with a single high-dose of radiation (90 Gy). Temporal changes in gene expression in the irradiated and non-irradiated neighboring lung regions were analyzed by microarray. For comparison, the long-term effect (12 months) of 20 Gy radiation on a diffuse region of lung was also measured. The majority of genes were down-regulated in the focally-irradiated lung areas at 2 to 3 weeks after irradiation. This pattern of gene expression was clearly different than gene expression in the diffuse region of lungs exposed to low-dose radiation. Ontological and pathway analyses indicated these down-regulated genes were mainly associated with organ development. Although the number was small, genes that were up-regulated after focal irradiation were associated with immune-relat...Continue Reading

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Citations

Jun 1, 2021·Cancer Treatment and Research Communications·Jana S HopstakenKoen J Hartemink

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Datasets Mentioned

BETA
GSE60541

Methods Mentioned

BETA
irradiating
Feature Extraction
PCR

Software Mentioned

Reactome FI
SPIA
Functional Annotation Tool of
Feature Extraction
Functional Annotation Tool Database for Annotation , Visualiza...
Signaling Pathway Impact Analysis ( SPIA )
DAVID
Cluster
REVIGO
Short Time - series Expression Miner ( STEM )

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