Focal stimulation of single GABAergic presynaptic boutons on the rat hippocampal neuron

Neuroscience Research
N AkaikeTakayoshi Imazawa

Abstract

Evoked inhibitory postsynaptic currents (eIPSCs) generated from a single GABAergic bouton were recorded and the functional properties were investigated. Native single boutons attached to mechanically dissociated rat hippocampal CA1 neurons, namely "synaptic bouton" preparation, were visualized with FM 1-43 dye and selectively stimulated by a glass pipette directed to a single bouton by focal stimulation. The GABAergic eIPSCs were elicited in like all-or-none fashion regarding both stimulus strength and pipette location, thus indicating that the eIPSCs result from the activation of a single bouton. The GABA release from the boutons was action potential dependent since eIPSCs were blocked in the presence of either voltage-dependent Na(+) or Ca(2+)channel blocker. Even in the presence of tetrodotoxin (TTX), eIPSCs could be elicited by additional application of a voltage-dependent K(+) channel blocker, 4-AP. The GABA release depended on external Ca(2+) concentration. Amplitude histogram of eIPSCs did not follow Poisson distribution or show discrete peaks. As a result, this new experimental approach using both focal stimulation and a synaptic bouton preparation allows for a detailed study of the native synaptic machinery in nerve te...Continue Reading

References

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Citations

Dec 25, 2002·Trends in Pharmacological Sciences·Norio Akaike, Andrew J Moorhouse
Apr 4, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Toshihiko Momiyama
Jun 10, 2011·Journal of Visualized Experiments : JoVE·Sang Beom JunDavid Lovinger
Aug 29, 2012·Brain Research Bulletin·Naoki Kotani, Norio Akaike
Jan 10, 2012·Toxicon : Official Journal of the International Society on Toxinology·Min-Chul ShinNorio Akaike
Feb 11, 2010·Toxicon : Official Journal of the International Society on Toxinology·Kiku NonakaNorio Akaike
Aug 19, 2009·Toxicology·Hironari AkaikeNorio Akaike
Oct 31, 2012·The Journal of Physiology·Norio AkaikeShunji Kozaki
Jun 20, 2012·The Journal of Physiology·Jesús Q Beltrán, Rafael Gutiérrez
May 12, 2015·Toxicon : Official Journal of the International Society on Toxinology·Masahito WakitaNorio Akaike
Dec 27, 2011·Journal of Pharmacological Sciences·Toshitaka YamagaNorio Akaike
Jul 6, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·O IevglevskyiO Krishtal
Aug 3, 2010·Toxicon : Official Journal of the International Society on Toxinology·Norio AkaikeRyuji Kaji
Sep 22, 2005·The Journal of Pharmacology and Experimental Therapeutics·Robert I Westphalen, Hugh C Hemmings
May 29, 2015·Journal of Neurophysiology·Masahito WakitaNorio Akaike
May 14, 2020·Journal of Neurophysiology·Il-Sung JangNorio Akaike
Jan 1, 2012·Journal of Pharmacological Sciences·Toshitaka YamagaNorio Akaike
Jul 1, 2020·Brain Research Bulletin·Michiko NakamuraNorio Akaike

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