Focusing on casein gene cluster and protein profile in Garganica goat milk

The Journal of Dairy Research
Marzia AlbenzioAgostino Sevi

Abstract

A survey was carried out in eight goat dairy farms, a total of 71 individual Garganica goat milk samples were collected for genomic DNA extraction. Casein alleles and haplotype frequencies of Garganica population were estimated. Individual milks were also analysed for chemical composition, rheological properties, and protein profile. The strong A* allele of CSN1S1 was predominant in the population investigated, the weak allele F of CSN1S1 showed a relatively high frequency and the null alleles N and 01 were first observed in this breed. At CSN1S2 locus the strong A* allele was the most frequent, followed by the F allele and the null allele. The strong A* allele was predominant at CSN2 locus, and relatively high incidence of null allele 0 was observed. CSN3 locus was monomorphic for B* allele. The exact test of sample differentiation based on haplotype frequencies discriminate the farms into two groups characterized by the highest frequency of strong (S-CSN1S1) or weak (W-CSN1S1) alleles at CSN1S1. Protein and casein contents were higher in the group characterized by strong allele than in the group with weak allele at CSN1S1. The 2D electrophoresis technique was performed to screen goat casein variability at the protein level an...Continue Reading

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Citations

Nov 6, 2009·The Journal of Dairy Research·Julia KüpperGeorg Erhardt
Jan 10, 2013·The Journal of Dairy Research·Filippo BaliaGiuseppe Massimo Vacca
May 21, 2010·Rapid Communications in Mass Spectrometry : RCM·Marina CuolloFrancesco Addeo
May 10, 2017·Foods·Marzia AlbenzioRosaria Marino
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Himani PuniaVirender S Mor

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