Folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway

Nutrients
Xing Gui LiaoJun Lin He

Abstract

It is well known that maternal folate deficiency results in adverse pregnancy outcomes. In addition to aspects in embryonic development, maternal uterine receptivity and the decidualization of stromal cells is also very important for a successful pregnancy. In this study, we focused on endometrium decidualization and investigated whether apoptosis, which is essential for decidualization, was impaired. Flow cytometry and TUNEL detection revealed that apoptosis of mouse endometrium decidual cells was suppressed in the dietary folate-deficient group on Days 7 and 8 of pregnancy (Day 1 = vaginal plug) when decidua regression is initiated. The endometrium decidual tissue of the folate deficiency group expressed less Bax compared to the normal diet group while they had nearly equal expression of Bcl2 protein. Further examination revealed that the mitochondrial transmembrane potential (ΔΨm) decreased, and the fluorescence of diffuse cytoplasmic cytochrome c protein was detected using laser confocal microscopy in normal decidual cells. However, no corresponding changes were observed in the folate-deficient group. Western blotting analyses confirmed that more cytochrome c was released from mitochondria in normal decidual cells. Taken to...Continue Reading

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Citations

Feb 20, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yan SuRu-Fei Gao
Apr 13, 2019·Molecular Human Reproduction·Xin YinJunlin He
Aug 1, 2021·Environmental Science and Pollution Research International·Yan ZhangXiaodan Wang

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Methods Mentioned

BETA
PCR
electrophoresis
fluorescence microscopy
FACS
transmission electron microscopy
flow cytometry

Software Mentioned

SPSS
Prism Graphpad
Statistical Package for the Social Sciences ( SPSS )
Quantity One

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis