Feb 19, 1999

Folate receptor type beta is a neutrophilic lineage marker and is differentially expressed in myeloid leukemia

Cancer
J F RossManohar Ratnam

Abstract

The membrane-associated folate receptor (FR) type beta is elevated in the spleen in patients with chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). In this study, the authors investigated possible cell type and differentiation stage specificity of expression of FR-beta in normal and leukemic hematopoietic cells. An affinity-purified rabbit polyclonal antibody specific for FR-beta was employed for immunostaining representative bone marrow smears and peripheral blood smears from normal individuals and from a limited number of patients with various leukemias. Multiple samples of normal bone marrow and peripheral blood were analyzed for the expression of FR-beta and selected CD antigens by two- or three-color flow cytometry. Of the morphologically identifiable cells, only neutrophils were positive for FR-beta. The leukemic blasts in CML patients showed expression of FR-beta with no apparent relation to the occurrence of the Philadelphia chromosome. Among acute nonlymphocytic leukemias, FR-beta was expressed in promyelocytic leukemia, in the myeloblast populations of myelomonocytic and erythroleukemias, and variably in M1/M2 AML. Neither the blasts of acute lymphocytic leukemia nor the more mature cells of chronic lym...Continue Reading

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  • Citations85

Citations

Mentioned in this Paper

Folate Receptor 2
Flow Cytometry
Myeloblast
Neutrophil Band Cells
PML gene
Leukocyte Differentiation Antigens, Human
CD19 Expressing Cell Count
Peripheral Blood
Spleen
Hormone Receptors, Cell Surface

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