Folate utilization by monomeric versus heterotetrameric sarcosine oxidases

Archives of Biochemistry and Biophysics
M A Wagner, Marilyn Schuman Jorns

Abstract

There are two types of bacterial sarcosine oxidases. The heterotetrameric enzymes contain subunits ranging in size from about 10 to 100 kDa, noncovalently bound FAD and NAD+, and covalently bound FMN attached to the beta subunit (42-45 kDa). Monomeric sarcosine oxidases are similar in size to the beta subunit in the heterotetramers and contain covalently bound FAD. Formaldehyde formation during sarcosine oxidation by several heterotetrameric sarcosine oxidases was suppressed in the presence of 50 microM [6S]-tetrahydrofolate, accompanied by a 25-50% increase in the rate of sarcosine oxidation. In contrast, [6S]-tetrahydrofolate caused only a modest decrease in the rate of formaldehyde production with monomeric sarcosine oxidases (approximately 25%), an effect which was virtually entirely attributable to an accompanying decrease in the rate of sarcosine oxidation. In the presence of 100 microM [6R,S]-tetrahydropteroyltriglutamate [H4Pte(Glu)3], the heterotetrameric enzymes catalyzed the formation of 5,10-methylenetetrahydropteroyltriglutamate [5,10-CH2-H4Pte(Glu)3] at a rate which was 35-60% faster than the rate of sarcosine oxidation in the absence of folate. An apparent Km value of 3.1 microM was estimated for [6S]-H4Pte(Glu)3...Continue Reading

References

Dec 1, 1985·Archives of Biochemistry and Biophysics·D H PorterC Wagner
Sep 1, 1988·Journal of Bacteriology·V E Worrell, D P Nagle
Dec 26, 1995·Biochemistry·A Willie, M S Jorns
Apr 23, 1996·Biochemistry·A WillieM S Jorns

❮ Previous
Next ❯

Citations

Sep 16, 2009·Biochimie·Laura CaldinelliLoredano Pollegioni
Mar 4, 2009·Biochimie·Mattia PedottiLoredano Pollegioni
Mar 26, 2003·European Journal of Biochemistry·Gianluca MollaLoredano Pollegioni
Aug 29, 2006·Journal of Molecular Biology·Robert M G HynsonMarilyn Schuman Jorns
Jun 1, 2005·Protein Expression and Purification·Alshaimaa Hassan-AbdallahMarilyn Schuman Jorns
Jun 11, 2005·Biochemical and Biophysical Research Communications·Koh IdaHaruo Suzuki
Feb 10, 2004·The Journal of Biological Chemistry·Aymeric GoyerAndrew D Hanson
Apr 24, 2004·The Journal of Biological Chemistry·Mario MörtlLoredano Pollegioni
Oct 28, 2015·Journal of Bacteriology·Graham G Willsey, Matthew J Wargo
Nov 16, 2011·Extremophiles : Life Under Extreme Conditions·Sangmin LeeGang-Won Cheong
Jul 14, 2000·Physiological Reviews·M Wyss, R Kaddurah-Daouk
Dec 18, 2001·The Journal of Biological Chemistry·Viviana JobLoredano Pollegioni
Apr 5, 2021·Archives of Biochemistry and Biophysics·Majd LahhamSilvia Wallner
Mar 13, 1999·Biochemistry·J BasranN S Scrutton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.