Folding and characterization of the amino-terminal domain of human tissue inhibitor of metalloproteinases-1 (TIMP-1) expressed at high yield in E. coli

FEBS Letters
W HuangK Brew

Abstract

Methods are described for producing an active amino-terminal domain of tissue inhibitor of metalloproteinases-1 (N-TIMP-1) from inactive protein expressed as inclusion bodies in E. coli. Yields exceed 20 mg per litre of bacterial culture. Activity measurements, CD spectroscopy and NMR spectroscopy of the 15N-labeled protein show that it is fully active, homogeneous in conformation and suitable for high-resolution structural analysis. The affinity of N-TIMP-1 for matrix metalloproteinases 1, 2 and 3 is 6-8-fold less than that of the recombinant full-length protein, indicating that deletion of the C-terminal domain reduces the free energy of interaction by < 10%.

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Citations

May 9, 2006·Cancer Metastasis Reviews·Rosemarie ChircoHyeong-Reh Choi Kim
Jun 1, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gregory S Makowski, Melinda L Ramsby
Mar 10, 2000·Biochimica Et Biophysica Acta·W Bode, R Huber
Mar 10, 2000·Biochimica Et Biophysica Acta·K BrewH Nagase
Aug 4, 2007·Journal of the American Chemical Society·Janelle Lauer-FieldsGregg B Fields
Oct 20, 2006·Molecular Biology of the Cell·Yoshifumi ItohMotoharu Seiki
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·W BodeK Maskos
Jul 23, 1999·Annals of the New York Academy of Sciences·H NagaseK Brew
Jun 1, 2013·Parasites & Vectors·Cinzia CantacessiAlex Loukas
Mar 14, 2003·Journal of Molecular Biology·S ArumugamSteven R Van Doren
Jul 18, 2006·Biochemical and Biophysical Research Communications·Antônio F M PintoJay W Fox
Jul 31, 2007·Protein Science : a Publication of the Protein Society·Asmaa B HamzeKeith Brew
Oct 26, 2011·Toxicon : Official Journal of the International Society on Toxinology·Hidetoshi InagakiTai Kubo
Nov 26, 2008·Molecular Cancer·Jorge Melendez-ZajglaVilma Maldonado
Oct 13, 2005·Biochemical and Biophysical Research Communications·L CrombezB Toussaint
Jun 22, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Maxim LevinIrit Sagi
Jul 11, 2008·Biopolymers·Steven R Van DorenKeith Brew
Feb 14, 2006·The Journal of Biological Chemistry·Jane L EnglishRama Khokha
Jan 25, 2008·The Biochemical Journal·Magdalini RaptiGillian Murphy
Jul 13, 2007·The Journal of Biological Chemistry·Janelle L Lauer-FieldsKeith Brew

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