PMID: 4332245Sep 1, 1971Paper

Folding of staphylococcal nuclease: magnetic resonance and fluorescence studies of individual residues

Proceedings of the National Academy of Sciences of the United States of America
H F EpsteinJ S Cohen


The reversible unfolding and folding of staphylococcal nuclease in the acid transition has been studied by 220 MHz proton magnetic resonance spectroscopy. The values of area, line-width, and chemical shift of each of the imidazole C2 proton resonances of the four histidine residues have been measured in this transition. The change of areas of three histidine resonances and the change of fluorescence of the single tryptophan residue, as a function of pH, appear to follow a single equilibrium. In contrast, a fourth histidine resonance follows a biphasic transition. These findings indicate that local conformational changes can be detected by magnetic resonance spectroscopy in the cooperative transition of the overall structure.


May 16, 1969·Annals of the New York Academy of Sciences·H EdelhochM Wilchek
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J S Cohen, O Jardetzky
Feb 6, 1970·Science·A N SchechterC B Anfinsen
Jul 13, 1970·Biochemical and Biophysical Research Communications·J S CohenA N Schnechter
Jan 13, 1971·Journal of the American Chemical Society·C C McDonaldJ D Glickson
Sep 13, 1969·Nature·J H Bradbury, N L King

❮ Previous
Next ❯


Oct 15, 1979·Experientia·H ShindoJ S Cohen
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D G Westmoreland, C R Matthews
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L P Yu, G M Smith
Dec 31, 1973·Annals of the New York Academy of Sciences·Y ArataO Jardetzky
Dec 31, 1973·Annals of the New York Academy of Sciences·C R Matthews, D G Westmoreland
Apr 14, 1976·Biochimica Et Biophysica Acta·A Grinvald, I Z Steinberg
Jan 1, 1978·Progress in Biophysics and Molecular Biology·T E Creighton
Jun 18, 2009·Proteins·Carlos A CastañedaBertrand E García-Moreno
Aug 15, 1974·Journal of Molecular Biology·T E Creighton
Apr 5, 1979·Journal of Molecular Biology·T E Creighton
Sep 5, 1983·Journal of Molecular Biology·K Kuwajima, R L Baldwin
Dec 31, 1973·Annals of the New York Academy of Sciences·G C Roberts, F W Benz
Jan 15, 1973·European Journal of Biochemistry·M H FreedmanJ S Cohen
Aug 1, 1984·Journal of Biomolecular Structure & Dynamics·S S Narula, M M Dhingra
Jul 20, 1973·Science·C B Anfinsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.