PMID: 4332245Sep 1, 1971Paper

Folding of staphylococcal nuclease: magnetic resonance and fluorescence studies of individual residues

Proceedings of the National Academy of Sciences of the United States of America
H F EpsteinJ S Cohen

Abstract

The reversible unfolding and folding of staphylococcal nuclease in the acid transition has been studied by 220 MHz proton magnetic resonance spectroscopy. The values of area, line-width, and chemical shift of each of the imidazole C2 proton resonances of the four histidine residues have been measured in this transition. The change of areas of three histidine resonances and the change of fluorescence of the single tryptophan residue, as a function of pH, appear to follow a single equilibrium. In contrast, a fourth histidine resonance follows a biphasic transition. These findings indicate that local conformational changes can be detected by magnetic resonance spectroscopy in the cooperative transition of the overall structure.

References

May 16, 1969·Annals of the New York Academy of Sciences·H EdelhochM Wilchek
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J S Cohen, O Jardetzky
Feb 6, 1970·Science·A N SchechterC B Anfinsen
Jul 13, 1970·Biochemical and Biophysical Research Communications·J S CohenA N Schnechter
Jan 13, 1971·Journal of the American Chemical Society·C C McDonaldJ D Glickson
Sep 13, 1969·Nature·J H Bradbury, N L King

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Citations

Oct 15, 1979·Experientia·H ShindoJ S Cohen
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D G Westmoreland, C R Matthews
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L P Yu, G M Smith
Dec 31, 1973·Annals of the New York Academy of Sciences·Y ArataO Jardetzky
Dec 31, 1973·Annals of the New York Academy of Sciences·C R Matthews, D G Westmoreland
Apr 14, 1976·Biochimica Et Biophysica Acta·A Grinvald, I Z Steinberg
Jan 1, 1978·Progress in Biophysics and Molecular Biology·T E Creighton
Jun 18, 2009·Proteins·Carlos A CastañedaBertrand E García-Moreno
Aug 15, 1974·Journal of Molecular Biology·T E Creighton
Apr 5, 1979·Journal of Molecular Biology·T E Creighton
Sep 5, 1983·Journal of Molecular Biology·K Kuwajima, R L Baldwin
Dec 31, 1973·Annals of the New York Academy of Sciences·G C Roberts, F W Benz
Jan 15, 1973·European Journal of Biochemistry·M H FreedmanJ S Cohen
Aug 1, 1984·Journal of Biomolecular Structure & Dynamics·S S Narula, M M Dhingra
Jul 20, 1973·Science·C B Anfinsen

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