PMID: 11342715May 9, 2001Paper

Folding screening assayed by proteolysis: application to various cystine deletion mutants of vascular endothelial growth factor

Protein Engineering
C Heiring, Y A Muller

Abstract

The production of recombinant proteins in Escherichia coli often leads to the formation of inclusion bodies. Although this has a number of advantages, a major disadvantage is the need to develop folding protocols for the renaturing of the proteins. However, the systematic screening of folding conditions is often hampered by the lack of convenient assays to detect correctly folded proteins. To address this problem we present a simple protocol, which combines folding screens and limited proteolysis to rapidly assess and optimize folding conditions. The efficacy of this method, termed FSAP (folding screening assayed by proteolysis), is demonstrated by the large-scale folding, purification and crystallization of various cystine deletion mutants of the cystine knot family member: vascular endothelial growth factor (VEGF). These mutants are particularly difficult to fold as the cystine knot is believed to make major contributions to the stability of the protein and this family of proteins lacks extensive hydrophobic core regions.

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Citations

Sep 11, 2002·Trends in Biotechnology·Anton P J Middelberg
Sep 12, 2001·Current Opinion in Biotechnology·U HeinemannH Oschkinat
Feb 18, 2010·PloS One·Carlene S Starck, Andrew J Sutherland-Smith
May 14, 2011·Biotechnology Journal·Bruno MantaAna Denicola
Dec 31, 2005·Protein Expression and Purification·Laura LinMark L Stahl
Sep 19, 2002·The Journal of Biological Chemistry·Yves A MullerHeinz Welfle
Aug 10, 2017·Biochemistry·Amy K SchaeferCatherine L Grimes

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