Folic acid-modified diatrizoic acid-linked dendrimer-entrapped gold nanoparticles enable targeted CT imaging of human cervical cancer

Journal of Cancer
Jiaying LinXinwu Lu

Abstract

It has been a great challenge to develop multifunctional fluorescent nanoprobes for tumor-targeted imaging. In this study, we developed folic acid (FA) gold nanoparticles (AuNPs) through diatrozic acid (DTA) linking forin vitroandin vivotargeted imaging of HeLa cervical cancer cells by computed tomography (CT). G5 dendrimers were used as templates to synthesize AuNPs within the interiors of dendrimers. The synthesized AuNPs were then sequentially modified by fluorescein isothiocyanate, FA, and DTA and the remaining terminal amines on the dendrimers were acetylated. We further performed hematoxylin and eosin staining, cell viability assay, flow cytometric analysis of cell cycle and apoptosis, and hemolytic assay to examine the cytotoxicity and hemocompatibility of the particles. The specific uptake of the nanoparticles by HeLa cells was determined through inductively coupled plasma atomic emission spectroscopy determination of silver and transmission electron microscopy. Lastly, HeLa cells and a xenografted HeLa tumor model were employed to evaluate thein vitroandin vivotargeted CT imaging performances of the nanoparticles, respectively. We showed that Au DENPs-FA-DTA does not cause cytotoxic effects on both HeLa cancer cells an...Continue Reading

Citations

May 23, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Jessica C HsuDavid P Cormode
Feb 16, 2019·Recent Patents on Drug Delivery & Formulation·Zeenat Iqbal, Fahima Dilnawaz
Mar 7, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Serge MignaniJean-Pierre Majoral
Mar 18, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Tingting XiaoXiangyang Shi

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Methods Mentioned

BETA
surgical resection
X-ray
acetylation
nuclear
transmission electron microscopy
xenograft
NMR
surface plasmon resonance
xenografts

Software Mentioned

FluoView
SPSS

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