Folic acid-modified mesoporous silica nanoparticles with pH-responsiveness loaded with Amp for an enhanced effect against anti-drug-resistant bacteria by overcoming efflux pump systems

Biomaterials Science
Xu ChenJie Liu

Abstract

Efflux pump system-mediated bacterial multidrug resistance is one of the main causes of antibiotic failure. Therefore, it is necessary to develop a novel nanocarrier that could effectively inhibit drug-resistant bacteria by increasing the intake and retention time of antibiotics. Herein, we constructed a pH-responsive nanocarrier (MSN@FA@CaP@FA) with double folic acid (FA) and calcium phosphate (CaP) covered on the surface of mesoporous silica (MSN) by electrostatic attraction and biomineralization, respectively. Afterward, loading the nanocomposites with ampicillin (Amp) effectively increased the uptake and reduced the efflux effect in Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by the specific targeting of FA. Moreover, Amp-MSN@FA@CaP@FA could specifically transport Amp to the bacterial infection site. Similarly, antibacterial experiments revealed that the Amp-MSN@FA@CaP@FA could significantly enhance the activity of Amp for inhibiting drug-resistant bacteria, without producing drug resistance. Additionally, the Amp-MSN@FA@CaP@FA could reduce the content of protein and inhibit the protein activity in drug-resistant bacteria, so that it destroyed the bacterial membrane and led to the bacteria death. In viv...Continue Reading

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Citations

Aug 8, 2020·Frontiers in Chemistry·Vanitha SelvarajanPui Lai Rachel Ee
Jun 11, 2020·Molecular Pharmaceutics·Shahriar SharifiMorteza Mahmoudi
Jan 14, 2021·Nanomaterials·John Jairo Aguilera-CorreaMaría Vallet-Regí
Nov 19, 2020·International Journal of Molecular Sciences·Montserrat Colilla, María Vallet-Regí
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Clara I ColinoCarmen Gutierrez-Millan
Feb 22, 2021·Drug Delivery and Translational Research·Pia KaiserMaike Windbergs
May 29, 2021·World Journal of Microbiology & Biotechnology·Ifeanyi E Mba, Emeka I Nweze
Jul 9, 2021·International Journal of Nanomedicine·Rafael R Castillo, María Vallet-Regí
Aug 11, 2021·Pharmaceutics·Elham RastegariYueh Chien

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Methods Mentioned

BETA
fluorescence imaging
flow cytometry
ELISA
atomic
force
AFM

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