Folium Sennae protects against hydroxyl radical-induced DNA damage via antioxidant mechanism: an in vitro study

Botanical Studies
Jian LinDongfeng Chen

Abstract

In the study, Folium Sennae (FS) was firstly extracted by various solvents to obtain five FS extracts. Then, five FS extracts were evaluated for the protective effects against •OH-induced DNA damage, antioxidant abilities in vitro, and chemical contents using various methods. On this basis, the correlation graphs between the pharmacological effects and chemical contents were plotted to obtain the correlation coefficients (R values). Finally, in order to obtain biological evidence, ethyl acetate extract of FS (EAFS) was investigated for the protective effect against •OH-induced MSCs (mesenchymal stem cells) damage using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl) assay. The pharmacological assays indicated that five FS extracts could effectively protect against •OH-induced DNA damage. The correlation analysis suggested that the average R values of total phenolics, total anthraquinones, aloe-emodin, rhein, and emodin were respectively 0.843, 0.833, 0.753, 0.820, and 0.784, while those of total sugars and total saponins were respectively 0.103 and 0.0068. The mechanistic analysis revealed that five FS extracts could also scavenge •OH, •O2(-), DPPH• & ABTS•(+) radicals, and reduce Cu(2+) to Cu(+). MTT assay revealed that the vi...Continue Reading

References

Aug 4, 1983·Nature·B Demple, J Halbrook
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Dec 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiLing Huang
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Sep 17, 2011·The Journal of Physical Chemistry. B·Cristina IugaAnnik Vivier-Bunge
Nov 15, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
Nov 24, 2012·Chemistry Central Journal·Xican LiDongfeng Chen
Mar 26, 2013·Analytical Biochemistry·Xican LiWeijuan Han

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Citations

Apr 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen
May 14, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xican LiDongfeng Chen

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS
Origin

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