Follicle-stimulating hormone affects metaphase I chromosome alignment and increases aneuploidy in mouse oocytes matured in vitro

Biology of Reproduction
Ruth RobertsKate Hardy

Abstract

Follicle-Stimulating Hormone (FSH) at a wide range of doses is routinely added to culture media during in vitro maturation (IVM) of oocytes, but the effects on oocyte health are unclear. The suggestion that superovulation may cause aneuploidy and fetal abnormalities prompted us to study the potential role of FSH in the genesis of chromosomal abnormalities during meiosis I. Mouse cumulus-oocyte complexes (COCs) isolated from the antral follicles of unprimed, sexually immature B6CBF1 mice were cultured in increasing concentrations of FSH. Following culture, matured oocytes were isolated, spread, stained with DAPI, and the numbers of chromosomes counted. Significantly increased aneuploidy, arising during the first meiotic division, was observed in metaphase II oocytes matured in higher concentrations of FSH (> or =20 ng/ml). The effect of FSH on spindle morphology and chromosome alignment during metaphase I was then explored using immunocytochemistry and three-dimensional reconstruction of confocal sections. High FSH had no effect on gross spindle morphology but did alter chromosome congression during prometaphase and metaphase, with the spread of chromosomes across the spindle at this time being significantly greater in oocytes c...Continue Reading

References

Nov 1, 1976·The Journal of Cell Biology·E Anderson, D F Albertini
Jan 1, 1992·Molecular Reproduction and Development·T A Rose, B D Bavister
Feb 1, 1990·Molecular Reproduction and Development·J J van de SandtJ J Eppig
Sep 1, 1974·Journal of Reproduction and Fertility·S FujimotoW R Dukelow
Jan 1, 1984·Annual Review of Genetics·T J Hassold, P A Jacobs
Apr 1, 1996·The Journal of Clinical Endocrinology and Metabolism·N SantoroJ H Skurnick
Jan 1, 1996·Environmental and Molecular Mutagenesis·U Eichenlaub-Ritter
Dec 20, 1996·Molecular and Cellular Endocrinology·P J O'ShaughnessyW R Rajapaksha
Nov 26, 1998·Reviews of Reproduction·L E YoungI Wilmut
May 18, 1999·Biology of Reproduction·S M Downs, A M Utecht
Dec 14, 1999·Journal of Medical Genetics·E R Maher
Feb 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·A TrounsonG Jones

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Citations

Feb 23, 2010·Journal of Assisted Reproduction and Genetics·Lynda K McGinnis, David F Albertini
Dec 15, 2010·Journal of Assisted Reproduction and Genetics·Marc-André Sirard
May 31, 2012·Journal of Assisted Reproduction and Genetics·Daniela Paes de Almeida Ferreira BragaEdson Borges
Aug 15, 2012·Journal of Assisted Reproduction and Genetics·I HammoudJ Selva
Feb 10, 2012·European Journal of Human Genetics : EJHG·Alan H HandysideJoep Geraedts
Dec 1, 2011·Reproduction, Fertility, and Development·Shan LiuAvner Hershlag
Jul 28, 2010·Human Reproduction·Sarah E HarrisHelen M Picton
Dec 19, 2008·Human Reproduction Update·M F G VerbergB C J M Fauser
Dec 1, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joanna Rutkowska, Alexander V Badyaev
Feb 13, 2008·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·E V DaevIa A Fil'kina
Aug 7, 2012·The Journal of Clinical Endocrinology and Metabolism·Elena LabartaAntonio Pellicer
Aug 28, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Margarida Avo SantosNick S Macklon
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Kimberly A PreisDavid K Gardner
May 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tomohiko AkiyamaFugaku Aoki
Oct 12, 2012·Journal of Assisted Reproduction and Genetics·Daniela Paes Almeida Ferreira BragaEdson Borges
Jan 9, 2013·Systems Biology in Reproductive Medicine·Steven D HorneHenry H Q Heng
Mar 8, 2012·Experimental Cell Research·Keith T Jones, Simon I R Lane
May 3, 2011·Reproductive Biomedicine Online·Tanya V SabhnaniJoyce C Harper
Sep 11, 2010·Fertility and Sterility·Jamie A M MassieRuth B Lathi
Jun 18, 2010·Fertility and Sterility·Yan-Wen XuCan-Quan Zhou
Aug 10, 2006·Reproductive Biomedicine Online·Ching-Chien ChangX Cindy Tian
Apr 14, 2009·Fertility and Sterility·Wei ShaQing-Yuan Sun
Dec 17, 2008·Seminars in Cell & Developmental Biology·Tobias Uller, Alexander V Badyaev
Jan 8, 2008·Fertility and Sterility·Jamie A M MassieRuth B Lathi
Sep 8, 2007·Gynécologie, obstétrique & fertilité·P Clément
Aug 21, 2013·American Journal of Medical Genetics. Part a·Ross RowseyTerry Hassold
Feb 13, 2007·Environmental Research·F PacchierottiJ B Mailhes

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Imaris
Imaris Surpass
GraphPad InStat
GraphPad

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