Follicular factors determining granulosa cell number and developmental competence of porcine oocytes

Journal of Assisted Reproduction and Genetics
Yasuhisa MunakataHisataka Iwata

Abstract

Granulosa cell (GC) number in follicles is a simple characteristic of follicles. The present study examined the hypothesis that follicular fluid (FF) determines GC number and oocyte developmental ability and revealed the molecular background determining the number of follicular GCs. FF was collected from antral follicles (3-5 mm in diameter), after which the number of GCs per follicle was determined for each donor gilt using real time PCR targeting single copy gene. GCs were analyzed by next-generation RNA sequencing and IPA pathway analysis. When oocyte cumulus cell-oocyte-complexes (COCs) were cultured in maturation medium containing 10% of each individual FF, the rate of development to the blastocyst stage was significantly correlated with the number of GCs in the donor gilt. In addition, when GCs were cultured in medium containing FF, the proliferative activity of the GCs was also significantly correlated to the number of GCs in the donor gilt. Moreover, when the FFs were categorized based on the number of GCs in the follicle, it was found that supplementation of culture media with GC-rich FF improved the developmental ability of oocytes compared to those supplemented with GC-poor FF. RNA sequencing and a pathway analysis o...Continue Reading

References

Dec 26, 2001·Biology of Reproduction·Koji YoshiokaShokichi Iwamura
Feb 10, 2006·Developmental Biology·Rabindranath De La Fuente
May 17, 2006·Zygote : the Biology of Gametes and Early Embryos·Masashi NaganoYoshiyuki Takahashi
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Hong-Thuy BuiTakashi Miyano
Apr 15, 2008·The Journal of Reproduction and Development·Koji YoshiokaAkira Onishi
Jul 31, 2009·Biology of Reproduction·Iris Manosalva, Aitor González
Jan 17, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Rachna MongaDheer Singh
Nov 7, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·M EndoH Iwata
Feb 2, 2016·Reproduction, Fertility, and Development·Shun TakeoHisataka Iwata
Jul 13, 2016·Theriogenology·Yasuhisa MunakataHisataka Iwata
Nov 20, 2016·Molecular Reproduction and Development·Yasuhisa MunakataHisataka Iwata
Sep 12, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Hugh J Clarke

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Citations

Aug 15, 2019·Zygote : the Biology of Gametes and Early Embryos·Kazuki KansakuHisataka Iwata
Aug 12, 2020·Journal of Assisted Reproduction and Genetics·Yuki InoueHisataka Iwata
Jul 18, 2019·Reproductive Medicine and Biology·Hidenori ShibaharaHisataka Iwata
Sep 15, 2020·The Journal of Reproduction and Development·Shuta NagataHisataka Iwata
May 11, 2021·Journal of Assisted Reproduction and Genetics·Takayuki YamochiYoshiharu Morimoto

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