Follistatin-like protein 1 promotes inflammatory reactions in nucleus pulposus cells by interacting with the MAPK and NFκB signaling pathways

Oncotarget
Yi LiuLei Cheng

Abstract

Follistatin-like protein 1 (FSTL1) is a well-known mediator of inflammation. Intervertebral disc disease is an inflammatory disorder. Here, we investigated the role of FSTL1 in the intervertebral discs inflammation. Expression of FSTL1 in nucleus pulposus tissues from rats and human was determined by immunohistochemistry staining and western blot analysis. The expression levels of tumor necrosis factor-α (TNF-α), interleukin1-β (IL-1β) and matrix metalloproteinase 13 (MMP-13) in human and rat nucleus pulposus tissues were measured by immunohistochemistry staining. The mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NFκB) signaling pathways were detected by western blotting. FSTL1 serum levels were significantly increased in lumbar disc herniation patients and had a positive correlation with Visual Analogue Scores. Additionally, FSTL1 expression was significantly increased in extrusion group compared with protrusion and control groups. Furthermore, FSTL1 expression was significantly increased in intervertebral disc degeneration models of rats. Immunohistochemistry staining demonstrated that the levels of TNF-α, IL-1β and MMP-13 were increased in the pathogenesis of intervertebral disc disease. Recombinant hum...Continue Reading

References

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Citations

Mar 30, 2018·Cellular and Molecular Life Sciences : CMLS·Andrea MattiottiMaurice J B van den Hoff
Aug 23, 2018·Oncotarget·Gerolamo BevivinoGiovanni Monteleone
Oct 6, 2017·Current Reviews in Musculoskeletal Medicine·Raj M AminBrian J Neuman
Oct 3, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaohe LiWen Ning
Jun 3, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yan WangLiang Dong
Aug 28, 2021·Biomedicines·Olga K ParfenovaDmitry V Grishin
Sep 11, 2021·Frontiers in Cell and Developmental Biology·Kaiwen LiuLei Cheng

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Methods Mentioned

BETA
enzyme-linked immune sorbent assay
ELISA
PCR
protein assay

Software Mentioned

ImageJ
SPSS
Pro Plus
Image

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