PMID: 9440870Jan 24, 1998Paper

Follow-up study of the revascularization process of purified rat islet beta-cell grafts

Cell Transplantation
J F MendolaR Gomis

Abstract

The revascularization of islets of Langerhans transplanted in heterotopic sites like the liver by portal vein embolization or the renal subcapsular space is a major process necessary for the viability of grafted cells. This process has been extensively studied by different techniques and the results have shown that islet revascularization is an early phenomenon that takes place soon after transplantation. In this report we have analyzed by a double indirect immunofluorescence technique, the revascularization process of purified endocrine islet beta-cells transplanted in the renal subcapsular space of syngeneic rats. Lewis rats were grafted with islets cultured for 24 h, with a suspension of purified beta-cells cultured for 24 h, and with a suspension of purified beta plus nonbeta-cells cultured for 24 h. Rats were killed at different days after implantation and the kidney bearing the grafts were snap frozen and immunohistochemically stained with a rabbit anti factor VIII antiserum (which labels endothelial cells). Immunocytochemical analysis revealed that cultured islets completed revascularization by days 3-5 after transplantation, as shown by the detection of capillary endothelial cells within and surrounding the islets. With...Continue Reading

Citations

Dec 9, 2010·Transplant International : Official Journal of the European Society for Organ Transplantation·Byung Wan LeeSung-Hee Ihm
Nov 30, 2000·Upsala Journal of Medical Sciences·P O CarlssonL Jansson
Jan 26, 2013·Transplant International : Official Journal of the European Society for Organ Transplantation·Hee Young ChaeSung-Hee Ihm

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