Following the differentiation of human pluripotent stem cells by proteomic identification of biomarkers

Stem Cells and Development
M W HaymanS A Przyborski

Abstract

Following the differentiation of cultured stem cells is often reliant on the expression of genes and proteins that provide information on the developmental status of the cell or culture system. There are few molecules, however, that show definitive expression exclusively in a specific cell type. Moreover, the reliance on a small number of molecules that are not entirely accurate biomarkers of particular tissues can lead to misinterpretation in the characterization of the direction of cell differentiation. Here we describe the use of technology that examines the mass spectrum of proteins expressed in cultured cells as a means to identify the developmental status of stem cells and their derivatives in vitro. This approach is rapid and reproducible and it examines the expression of several different biomarkers simultaneously, providing a profile of protein expression that more accurately corresponds to a particular type of cell differentiation.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G S EisenbarthM Nirenberg
May 1, 1997·Differentiation; Research in Biological Diversity·K FröjdmanJ E Eriksson
Jun 5, 1999·Biotechnology Progress·V HatzimanikatisK H Lee
Oct 13, 2000·The European Journal of Neuroscience·S A PrzyborskiP W Andrews
Feb 28, 2002·Lancet·Emanuel F PetricoinLance A Liotta
Apr 2, 2002·Biochemical and Biophysical Research Communications·Haleem J IssaqDonna Felschow
May 30, 2002·Journal of Anatomy·Jonathan S DraperPeter W Andrews
Sep 14, 2002·Science·Miguel Ramalho-SantosDouglas A Melton
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Dec 10, 2003·Experimental Hematology·Richard D UnwinAnthony D Whetton
Jan 7, 2004·Mechanisms of Development·Alexandra DelacourPedro Luis Herrera
Mar 23, 2004·Biochemical and Biophysical Research Communications·M W Hayman, S A Przyborski
Apr 23, 2004·Current Opinion in Biotechnology·Emanuel F Petricoin, Lance A Liotta
Apr 27, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jonathan TolsonThomas Flad
Jul 20, 2004·Disease Markers·Christine LarongaO John Semmes
Jul 31, 2004·World Journal of Gastroenterology : WJG·Xiao-Dong ZhuPo Tien
Sep 4, 2004·Stem Cells and Development·S A PrzyborskiG M Horrocks
Sep 17, 2004·Biochemical and Biophysical Research Communications·John D CalhounSteven L Stice
Oct 20, 2004·Biotechnology and Bioengineering·Raj R RaoSteven L Stice
Oct 27, 2004·Disease Markers·Brian A StanleyJennifer E Van Eyk
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·C WieseA M Wobus
Jan 18, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Seung-won LeeJin Young Kim
Jan 25, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·Irina A BuhimschiCatalin S Buhimschi
Feb 15, 2005·Biochemical and Biophysical Research Communications·Christoph ZenzmaierAndreas J Kungl
Jun 1, 2005·Cancer Letters·Valérie CombaretAlain Puisieux
Aug 10, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ulrich RauchKarl-Herbert Schäfer

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