FolX from Pseudomonas aeruginosa is octameric in both crystal and solution.

FEBS Letters
Mads GabrielsenAndrew J Roe

Abstract

FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs. Here we report the crystal structure of FolX from the bacterial opportunistic pathogen Pseudomonas aeruginosa, as well as a detailed analysis of the protein in solution, using analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS). In combination, these techniques confirm that the protein is an octamer both in the crystal structure, and in solution.

References

Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jan 1, 1993·Protein Engineering·G J Barton
Jun 1, 1997·Nature Structural Biology·A AchariD K Stammers
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Andrew G W Leslie
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Apr 25, 2007·Nucleic Acids Research·Ian W DavisDavid C Richardson
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Dec 21, 2007·Acta Crystallographica. Section D, Biological Crystallography·Fei LongGarib N Murshudov
Apr 25, 2009·Acta Crystallographica. Section D, Biological Crystallography·Charles Simon Bond, Alexander Wolfgang Schüttelkopf
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Dec 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Mads GabrielsenAndrew J Roe
Apr 1, 2009·Journal of Applied Crystallography·Daniel Franke, Dmitri I Svergun

❮ Previous
Next ❯

Citations

Dec 19, 2014·Chemical Biology & Drug Design·Riccardo ZambelloniAndrew J Roe
Jul 21, 2015·Computational Biology and Chemistry·Utku DenizElif Ozkirimli
Apr 10, 2018·Future Medicinal Chemistry·Marcio V Bertacine DiasSair M Chavez-Pacheco

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray

Software Mentioned

MOLPROBITY
BUSTER
PRIMUS
ALSCRIPT
GNOM
COOT
SOMO
SCALA
Origin
MOSFLM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.