Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus)

PloS One
Tassiana Gutierrez de PaulaMaeli Dal-Pai-Silva

Abstract

Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1α and SDHA), together with a decrease in PPARβ/δ mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar p...Continue Reading

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Citations

Apr 5, 2018·Frontiers in Genetics·Marcos E HerkenhoffDanillo Pinhal
Jan 11, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hélène Alami-DuranteSadasivam Kaushik
Apr 4, 2021·International Journal of Molecular Sciences·Bruna Tereza Thomazini ZanellaMaeli Dal-Pai-Silva

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

R Bioconductor
Geneious
gplots
Primer Express
heatmap .
Image Analysis System

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