Foot formation in Hydra: a novel gene, anklet, is involved in basal disk formation

Mechanisms of Development
Yasuko AmimotoYoshitaka Kobayakawa

Abstract

We isolated a novel gene by a differential-display RT-PCR method comparing basal disk tissue and peduncle tissue in a species of Hydra, Pelmatohydra robusta, and we referred to it as anklet. The putative anklet product has a signal sequence in its N-terminus, and it has one MAC/PF domain and one EGF domain. In normal hydra, the expression of anklet was restricted in the periphery of the basal disk and the lowest region of the peduncle. In foot-regenerating animals, anklet was first expressed in the newly differentiated basal disk gland cells at the regenerating basal end, and then expression became restricted at the periphery of the regenerated basal disk and in the lowest region of the peduncle. This spatially specific expression pattern suggested that the product of the anklet gene plays a role in basal disk formation. We therefore examined the role played by the protein product of the anklet gene by suppressing the transcription level of anklet using an RNA-mediated interference (RNAi) method. Suppression of the level of expression of the anklet gene led to a decrease in basal disk size in normal hydra, and to a delay in basal disk regeneration in foot-amputated animals. These results suggested that anklet is involved in the...Continue Reading

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Citations

Mar 25, 2009·Toxicon : Official Journal of the International Society on Toxinology·Daniel Sher, Eliahu Zlotkin
Dec 19, 2006·Molecular Phylogenetics and Evolution·Georg HemmrichThomas C G Bosch
Apr 29, 2014·Toxicon : Official Journal of the International Society on Toxinology·Eliezra GlasserDaniel Sher
Dec 19, 2014·Molecular Biology and Evolution·Tamar RachamimDaniel Sher
Mar 14, 2009·Tsitologiia·V N ShirokovaM P Samoĭlovich
Aug 8, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel SherEliahu Zlotkin

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