PMID: 6412211Jun 1, 1983Paper

Force and force transients in skeletal muscle fibres of the frog skinned by freeze-drying

Pflügers Archiv : European journal of physiology
G J StienenJ C Rüegg

Abstract

A freeze-drying method is described by which single skinned skeletal muscle fibres or fibre bundles can readily be obtained. Skinned fibre segments of the ileofibularis and semitendinous muscles of the frog - activated by means of a rapid increase in the Ca-concentration - showed very stable and reproducible contractions. Complete activation occurred at a Ca-concentration of 1.6 X 10(-6) M and the mid-point of the pCa-tension curve occurred at 6.3 X 10(-7) M. Addition of phosphate (less than or equal to 10(-2) M) had a depressing effect on the speed of the Ca-activated tension development as well as on the maximum tension reached. Addition of caffeine (10(-2) M) had no effect on the tension generation, indicating that the sarcoplasmic reticulum, if present, was not active. The force responses due to rapid length changes applied to the Ca-activated fibre preparations were found to be qualitatively similar to the force responses on intact tissue. This skinning technique might be employed on human biopsies, enabling the measurement of physiological parameters such as for example force and shortening velocity.

References

May 15, 1979·Pflügers Archiv : European journal of physiology·S P Robertson, W G Kerrick
Jan 1, 1971·Pflügers Archiv : European journal of physiology·J C RüeggG Müller
Jan 1, 1981·The American Journal of Physiology·E W Stephenson
Jan 1, 1981·Pflügers Archiv : European journal of physiology·J W HerzigJ C Rüegg
Jan 1, 1980·Biophysics of Structure and Mechanism·P J GriffithsJ C Rüegg
Aug 1, 1981·The Journal of General Physiology·G J Stienen, T Blangé
Jun 1, 1982·The Journal of General Physiology·P W BrandtT Robinson
Mar 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·H Mueller

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Citations

Sep 1, 1985·Pflügers Archiv : European journal of physiology·G J Stienen, T Blangé
Sep 1, 1985·Pflügers Archiv : European journal of physiology·G J StienenB W Treijtel
Nov 1, 1986·Pflügers Archiv : European journal of physiology·K Güth, R Wojciechowski
Jun 1, 1993·Journal of Muscle Research and Cell Motility·M E De WinkelB W Treijtel
Apr 1, 1992·Pflügers Archiv : European journal of physiology·D W JungB W Treijtel
Apr 1, 1994·Journal of Muscle Research and Cell Motility·M E De WinkelB W Treijtel
Jan 1, 1987·Pflügers Archiv : European journal of physiology·G J StienenG Elzinga
Aug 16, 2000·The Journal of Physiology·G J Stienen
Jul 20, 2018·Journal of Applied Physiology·Graham D Lamb, D George Stephenson
Jan 1, 1993·Cell Motility and the Cytoskeleton·K TrombitásK Wang
Apr 1, 1988·The American Journal of Physiology·E L de BeerP Schiereck
Sep 8, 1998·Journal of Applied Physiology·M P SlawnychB H Bressler
Apr 30, 1998·Physiological Reviews·B M Millman
Mar 1, 1991·The American Journal of Physiology·R L GründemanP Schiereck

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