Force-feedback high-speed atomic force microscope for studying large biological systems

Micron : the International Research and Review Journal for Microscopy
Byung I Kim, Ryan D Boehm

Abstract

We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.

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Citations

Aug 10, 2013·The Journal of Chemical Physics·Byung I KimJeremy R Bonander
Sep 4, 2012·The Review of Scientific Instruments·O D PaytonA R Champneys
Jan 1, 2013·Ultramicroscopy·Byung I Kim, Ryan D Boehm
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Jan 20, 2015·Nanotechnology·P KlapetekM Miles
Jan 13, 2021·Sensors·Luke Oduor OtienoYong Joong Lee

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