Force generation by endocytic actin patches in budding yeast

Biophysical Journal
A E Carlsson, P V Bayly


Membrane deformation during endocytosis in yeast is driven by local, templated assembly of a sequence of proteins including polymerized actin and curvature-generating coat proteins such as clathrin. Actin polymerization is required for successful endocytosis, but it is not known by what mechanisms actin polymerization generates the required pulling forces. To address this issue, we develop a simulation method in which the actin network at the protein patch is modeled as an active gel. The deformation of the gel is treated using a finite-element approach. We explore the effects and interplay of three different types of force driving invagination: 1), forces perpendicular to the membrane, generated by differences between actin polymerization rates at the edge of the patch and those at the center; 2), the inherent curvature of the coat-protein layer; and 3), forces parallel to the membrane that buckle the coat protein layer, generated by an actomyosin contractile ring. We find that with optimistic estimates for the stall stress of actin gel growth and the shear modulus of the actin gel, actin polymerization can generate almost enough force to overcome the turgor pressure. In combination with the other mechanisms, actin polymerizat...Continue Reading


Apr 1, 1994·Journal of Biomechanics·E K RodriguezA D McCulloch
Jan 6, 1999·Biophysical Journal·Andreas R BauschE Sackmann
Aug 31, 1999·Biophysical Journal·V C AbrahamF Lanni
Jul 6, 2000·European Biophysics Journal : EBJ·F GerbalJ Prost
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Arpita UpadhyayaAlexander van Oudenaarden
Jun 20, 2003·Current Biology : CB·Avital A RodalDavid G Drubin
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yann MarcyCécile Sykes
Jul 13, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Hakim BoukellalCécile Sykes
Sep 1, 2004·The Journal of Cell Biology·Michael E YoungPaul C Bridgman
Feb 16, 2005·Nature Cell Biology·Jiro ToshimaDavid G Drubin
Mar 24, 2005·Physical Review Letters·Falk WottawahJosef Käs
Jun 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Huajian GaoLambert B Freund
Nov 15, 2005·Biophysical Journal·Sean X Sun, Denis Wirtz
May 26, 2006·Nature Reviews. Molecular Cell Biology·Marko KaksonenDavid G Drubin
May 30, 2006·Biophysical Journal·Richard B Dickinson, Daniel L Purich
Dec 6, 2006·The Journal of Chemical Physics·Vagelis A Harmandaris, Markus Deserno
Dec 23, 2006·The European Physical Journal. E, Soft Matter·J Zhu, A E Carlsson
May 4, 2007·Molecular Biology of the Cell·Derek A ApplewhiteGary G Borisy
Mar 19, 2008·The Journal of Cell Biology·Fatima-Zahra IdrissiMaría-Isabel Geli
Feb 3, 2009·Current Opinion in Cell Biology·Brian J Galletta, John A Cooper
Jun 6, 2009·Ageing Research Reviews·Fangbai Wu, Pamela J Yao
Jul 15, 2009·Nature Cell Biology·Soheil Aghamohammadzadeh, K R Ayscough
Oct 2, 2009·HFSP Journal·Jean-François Joanny, Jacques Prost
Jul 1, 2010·Molecular Biology of the Cell·Vladimir SirotkinThomas D Pollard
Jul 2, 2010·Journal of Biomechanical Engineering·Gang XuLarry A Taber
Jul 20, 2010·Current Opinion in Biotechnology·Brian J GallettaJohn A Cooper
Jun 7, 2011·Biophysical Journal·Todd ThoresenMargaret L Gardel
Jul 5, 2011·Current Biology : CB·Agnieszka CollinsTatyana Svitkina
Oct 27, 2011·Physical Review Letters·Nilushi L DasanayakeA E Carlsson
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fatima-Zahra IdrissiMaria Isabel Geli
Dec 4, 2012·Biophysical Journal·Brian J GallettaJohn A Cooper
Apr 19, 2013·Physical Biology·Nilushi L Dasanayake, A E Carlsson


Feb 5, 2015·Biophysical Journal·Tao ZhangJ M Schwarz
Mar 24, 2016·The Journal of Cell Biology·Serge Dmitrieff, François Nédélec
Mar 31, 2016·Biophysical Journal·Xinxin WangA E Carlsson
Apr 22, 2015·Developmental Cell·Michal SkruznyMarko Kaksonen
Oct 31, 2015·PLoS Computational Biology·Serge Dmitrieff, François Nédélec
Feb 8, 2018·Nature Reviews. Molecular Cell Biology·Marko Kaksonen, Aurélien Roux
Dec 15, 2017·PLoS Computational Biology·Xinxin Wang, A E Carlsson
Jul 15, 2018·FEBS Letters·Michael M LacyJulien Berro
Jul 7, 2019·Cytoskeleton·Rui Ma, Julien Berro
Aug 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wenna ZhengYan Guo
Nov 21, 2019·Physical Biology·F Motahari, A E Carlsson
Nov 21, 2018·Life Science Alliance·Alexander E EpsteinThomas D Pollard
Jun 18, 2017·Physical Review. E·D J TwetenA E Carlsson
Jul 8, 2017·Molecular Biology of the Cell·Fred Chang
Jun 27, 2019·Molecular Biology of the Cell·Masoud NickaeenBoris Slepchenko
Feb 7, 2015·Genetics·Bruce L GoodeBeverly Wendland
Jan 28, 2017·Proceedings of the National Academy of Sciences of the United States of America·Julian E HassingerPadmini Rangamani
Sep 26, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K E GarciaP V Bayly
Apr 25, 2018·Nature Communications·Katharina DürreAndreas R Bausch
Mar 12, 2021·Current Opinion in Cell Biology·Christopher T LeePadmini Rangamani
Mar 25, 2021·ACS Nano·Yi ShenTuomas P J Knowles

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.