Force measurements on single molecular contacts through evanescent wave microscopy

Biophysical Journal
Giovanni Zocchi

Abstract

We introduce a new method to apply controlled forces on single molecules. The motion of a micron-sized bead attached to a solid surface through a single molecular contact is tracked by evanescent wave microscopy as a force is exerted through a flow. We report measurements of the streptavidin-biotin bond rupture force obtained with this technique. We also obtain detailed measurements of the balance of forces involved in detaching an adhering bead with a flow. A small lateral force translates into a much bigger normal force on the attachment point. This effect is relevant for the interpretation of common cell adhesion assays.

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Citations

Feb 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Björn M ReinhardJan Liphardt
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mukta Singh-ZocchiGiovanni Zocchi
Sep 30, 2004·Biophysical Journal·Sébastien PierratPierre Nassoy
Sep 27, 2002·Biophysical Journal·Mukta Singh-ZocchiGiovanni Zocchi
Apr 22, 2006·The Journal of Chemical Physics·Artur B Adib
Mar 4, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Giovanni Zocchi
Jun 20, 2003·Current Opinion in Nephrology and Hypertension·Robert L BacallaoCarrie L Phillips
Jul 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Matthias SchickingerHendrik Dietz
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Jeungphill HanneKim Ø Rasmussen
May 21, 2005·Physical Review Letters·Sanhita DixitGiovanni Zocchi
Jan 18, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Volkmar HeinrichEvan Evans
Dec 3, 2011·Cold Spring Harbor Protocols·Alexander R Dunn, James A Spudich

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