PMID: 9557705Apr 29, 1998Paper

Forced evolution reveals the importance of short open reading frame A and secondary structure in the cauliflower mosaic virus 35S RNA leader

Journal of Virology
M M PoogginJ Fütterer

Abstract

Cauliflower mosaic virus pregenomic 35S RNA begins with a long leader sequence containing an extensive secondary structure and up to nine short open reading frames (sORFs), 2 to 35 codons in length. To test whether any of these sORFs are required for virus viability, their start codons were mutated either individually or in various combinations. The resulting viral mutants were tested for infectivity on mechanically inoculated turnip plants. Viable mutants were passaged several times, and the stability of the introduced mutations was analyzed by PCR amplification and sequencing. Mutations at the 5'-proximal sORF A and in the center of the leader resulted in delayed symptom development and in the appearance of revertants. In the central leader region, the predicted secondary structure, rather than the sORF organization, was restored, while true reversions or second-site substitutions in response to mutations of sORF A restored this sORF. Involvement of sORF A and secondary structure of the leader in the virus replication cycle, and especially in translation of the 35S RNA via ribosome shunting, is discussed.

References

Jan 1, 1991·Genes & Development·H SanfaçonT Hohn
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Aug 1, 1980·Cell·A FranckL Hirth
Oct 20, 1995·Virology·J M Mesnard, C Carriere
Apr 1, 1994·Trends in Biochemical Sciences·A P Geballe, D R Morris
Oct 1, 1993·Plant Molecular Biology·M J Dowson DayR A Dixon
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R D Damiani, S R Wessler
Jan 1, 1995·Virus Genes·R Hull, S N Covey
Dec 1, 1996·Molecular and Cellular Biology·J Cao, A P Geballe
Dec 1, 1996·Trends in Microbiology·Z Kiss-László, T Hohn
Jul 1, 1997·The Journal of General Virology·D B SmithP Simmonds

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Citations

Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·M M PoogginT Hohn
Jan 16, 2008·Molecular and Cellular Biology·Kyle W Sherrill, Richard E Lloyd
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·R CuestaR J Schneider
Nov 9, 2006·Nucleic Acids Research·Todd BlevinsMikhail M Pooggin
Apr 5, 2000·The Journal of Biological Chemistry·M M PoogginJ Fütterer
Feb 9, 2000·Journal of Virology·O Guerra-PerazaM Hemmings-Mieszczak
Nov 1, 2002·Molecular Plant Pathology·Muriel HaasMario Keller
Jul 20, 2000·Micron : the International Research and Review Journal for Microscopy·T HohnL Ryabova
Dec 6, 2005·Virus Research·Lyubov A RyabovaThomas Hohn

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